Invitrogen

CDK2 Monoclonal Antibody (1A6)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (61) CDK2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CDK2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of HeLa cells using CDK2 monoclonal antibody (Product # MA5-17052) (Green). Red: actin filaments have been labeled with phalloidin.

CDK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CDK2 monoclonal antibody (Product # MA5-17052) followed with DAB staining.

CDK2 Antibody in Western Blot (WB) — Knockout of CDK2 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR692361_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of CDK2 was performed by loading 30 µg of A-431 wild type (Lane 1), A-431 Cas9 (Lane 2) andA-431 CDK2 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-CDK2 Monoclonal Antibody (1A6) (Product # MA5-17052, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:5000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to CDK2. An uncharacterized band was observed at ~55 kDa in all the samples.

CDK2 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells using CDK2 monoclonal antibody (Product # MA5-17052) (green) and negative control (red).

CDK2 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inCDK2 KO cell line compared to control cell line using Anti-CDK2 Monoclonal Antibody (1A6) (Product # MA5-17052).

Product Details

MA5-17052

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200 - 1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1A6

Immunogen

Purified recombinant fragment of human CDK2 expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2538524

Product Specific Information

MA5-17052 targets CDK2 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17052 immunogen is purified recombinant fragment of human CDK2 expressed in E. Coli.

MA5-17052 detects CDK2 which has a predicted molecular weight of approximately 33.9kDa.

Target Information

p33cdk2 associates with cyclin A in human cells. Kinase activity associated with cyclin A-cdc2 is found only in G2-phase. Cdk2 also complexes with cyclins E, D1, and D3. Cyclin E-cdk2 kinase is active in the G1- and S-phases of the cell cycle and is important (as does cyclin A-cdk2) for the progression from G1-to S-phase. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer.

Cancers

Hermanowicz JM,Pawlak K,Sieklucka B,Czarnomysy R,Kwiatkowska I,Kazberuk A,Surazynski A,Mojzych M,Pawlak D

MA5-17052 was used in Western Blot to examine the pharmacodynamics of MM-129 as a novel promising drug candidate against colon cancer.

Sat Jun 26 00:00:00 EDT 2021

MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer.

Cancers

Hermanowicz JM,Pawlak K,Sieklucka B,Czarnomysy R,Kwiatkowska I,Kazberuk A,Surazynski A,Mojzych M,Pawlak D

MA5-17052 was used in Western Blot to examine the pharmacodynamics of MM-129 as a novel promising drug candidate against colon cancer.

Sat Jun 26 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; Cell division protein kinase 2; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; p33 protein kinase; p33CDK2

Gene Aliases: A630093N05Rik; CDK2; CDKN2; p33(CDK2)

UniProt ID: (Human) P24941, (Mouse) P97377

Entrez Gene ID: (Human) 1017, (Mouse) 12566

Molecular Function: non-receptor serine/threonine protein kinase