Abnova

CDK4 Monoclonal Antibody (2G7)

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CDK4 Antibody in Western Blot (WB) — CDK4 monoclonal antibody (M04), clone 2G7 Western Blot analysis of CDK4 expression in HeLa (Cat # L013V1).

CDK4 Antibody in Immunocytochemistry (ICC) — Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. Antibody concentration is 10 µg/mL.

CDK4 Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged CDK4 is approximately 0.1 ng/mL as a capture antibody.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

Immunofluorescence (IF)

10 µg/mL

ELISA (ELISA)

0.1 ng/mL

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2G7

Immunogen

CDK4 (AAH03644, 211 a.a. approximately 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: KPLFCGNSEA DQLGKIFDLI GLPPEDDWPR DVSLPRGAFP PRGPRPVQSV VPEMEESGAQ LLLEMLTFNP HKRISAFRAL QHSYLHKDEG NPE

Target Information

CDK4 is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of the gene have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cell division protein kinase 4; CRK3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; MGC14458; p34/cdk4; PSK-J3; serine/threonine kinase

Gene Aliases: CDK4; CMM3; Crk3; PSK-J3

UniProt ID: (Human) P11802, (Mouse) P30285

Entrez Gene ID: (Human) 1019, (Mouse) 12567

Molecular Function: kinase non-receptor serine/threonine protein kinase non-receptor tyrosine protein kinase protein kinase transferase