Invitrogen

CDK5 Monoclonal Antibody (4E4)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (25) CDK5 antibodies

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CDK5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CDK5 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CDK5 Monoclonal Antibody (4E4) (Product # MA5-17053) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing predominant nuclear localization. The images were captured at 60X magnification.

CDK5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of GC7901 cells using CDK5 monoclonal antibody (Product # MA5-17053) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

CDK5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded cerebellum tissues (left) and brain tissues (right) using CDK5 monoclonal antibody (Product # MA5-17053) followed with DAB staining.

CDK5 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), PC-3 (Lane 2), HeLa (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), A549 (Lane 6), Jurkat (Lane 7), tissue extracts of Mouse Brain (Lane 8) and Rat Brain (Lane 9). The blot was probed with CDK5 Monoclonal Antibody (Product # MA5-17053, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A band at 33kDa corresponding to CDK5 was observed across the cell lines and tissues tested.

CDK5 Antibody in Western Blot (WB) — Western blot analysis of CDK5 was performed by loading 30 ug of HEK293 control (Lane 1), HEK293- CDK5 knockout (Lane 2) edigene lysates. The blot was probed with CDK5 Monoclonal Antibody (4E4) (Product # MA5-17053, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to CDK5.

CDK5 Antibody in Western Blot (WB) — Western blot analysis of CDK5 using CDK5 monoclonal antibody (Product # MA5-17053) in HeLa (1), K562 (2), PC-12 (3) and COS-7 (4) cell lysate.

CDK5 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of K562 cells using CDK5 monoclonal antibody (Product # MA5-17053) (green) and negative control (purple).

CDK5 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in CDK5 knockout (KO) cell line compared to control cell line using CDK5 Monoclonal Antibody (4E4) (Product #MA5-17053). {KO}

Product Details

MA5-17053

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:200 - 1:400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	4E4
Immunogen	Purified recombinant fragment of human CDK5 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.03% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2538525

Product Specific Information

MA5-17053 targets CDK5 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Non-human primate, and Rat samples.

The MA5-17053 immunogen is purified recombinant fragment of human CDK5 expressed in E. Coli.

MA5-17053 detects CDK5 which has a predicted molecular weight of approximately 35kDa.

Target Information

Cyclin-dependent kinase-5 (CDK5) is a member of the cyclin-dependent kinase family of serine/threonine kinases. Its mRNA and protein are expressed in the kidneys, testes, and ovaries. And Its activity has been detected almost exclusively in brain extracts. Similar to other Cdks, monomeric Cdk5 displays no enzymatic activity; however, Cdk5 is not activated bycyclins. Instead, Cdk5 activity requires association with one of two brain-specific regulatory subunits, called p35 and p39. These two activators regulate the spatial and temporal expression of active Cdk5, restricting its activity primarily to post-mitotic neurons.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CDK5-SV; Cell division protein kinase 5; CR6 protein kinase; Cyclin-dependent kinase 5; Cyclin-dependent-like kinase 5; protein kinase CDK5 splicing; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit

Gene Aliases: AW048668; CDK5; CDKN5; Crk6; LIS7; PSSALRE

UniProt ID: (Human) Q00535, (Mouse) P49615, (Rat) Q03114

Entrez Gene ID: (Human) 1020, (Mouse) 12568, (Rat) 140908

Molecular Function: non-receptor serine/threonine protein kinase

neuron migration synaptic transmission, dopaminergic intracellular protein transport nucleocytoplasmic transport cell cycle cell-matrix adhesion chemical synaptic transmission synapse assembly skeletal muscle tissue development motor neuron axon guidance cell proliferation visual learning response to wounding Schwann cell development synaptic vesicle exocytosis regulation of macroautophagy phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation sensory perception of pain cerebellar cortex formation hippocampus development layer formation in cerebral cortex central nervous system neuron development corpus callosum development neuron differentiation regulation of cell migration negative regulation of axon extension cortical actin cytoskeleton organization neuron projection development negative regulation of protein ubiquitination negative regulation of synaptic plasticity positive regulation of protein binding receptor catabolic process serine phosphorylation of STAT3 protein synaptic transmission, glutamatergic protein localization to synapse regulation of apoptotic process receptor clustering positive regulation of neuron apoptotic process negative regulation of cell cycle positive regulation of protein kinase activity negative regulation of proteolysis negative regulation of transcription, DNA-templated positive regulation of calcium ion-dependent exocytosis protein autophosphorylation negative regulation of protein export from nucleus behavioral response to cocaine regulation of synaptic plasticity synaptic vesicle endocytosis rhythmic process axon extension oligodendrocyte differentiation dendrite morphogenesis cell division neuron apoptotic process excitatory postsynaptic potential regulation of dendritic spine morphogenesis calcium ion import regulation of cell cycle arrest positive regulation of protein targeting to membrane negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of synaptic vesicle recycling positive regulation of actin cytoskeleton reorganization positive regulation of receptor activity protein phosphorylation exocytosis apoptotic process nervous system development axonogenesis associative learning cell migration telencephalon development cerebellum development cerebellar cortex development cerebral cortex development forebrain development response to cocaine regulated exocytosis neuron projection morphogenesis regulation of cell cycle regulation of postsynaptic membrane potential central nervous system development

Process(es)

p53 binding protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity ErbB-2 class receptor binding protein binding ATP binding cytoskeletal protein binding kinase activity protein kinase binding acetylcholine receptor activator activity ErbB-3 class receptor binding ephrin receptor binding tau-protein kinase activity nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups