Invitrogen

CDK6 Recombinant Rabbit Monoclonal Antibody (SD20-50)

Product Image Gallery

CDK6 Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of CDK6 in SW480 cells using a CDK6 Monoclonal antibody (Product # MA5-32357) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

CDK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CDK6 of paraffin-embedded Human colon cancer tissue using a CDK6 Monoclonal antibody (Product #MA5-32357). Counter stained with hematoxylin.

CDK6 Antibody in Western Blot (WB) — Western blot analysis of CDK6 in different lysates using a Monoclonal antibody (Product #MA5-32357) at a dilution of 1:1,000. Positive control: Lane 1: Jurkat, Lane 2: K562, Lane 3: Hela.

CDK6 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of CDK6 in Hela cells using a CDK6 Monoclonal Antibody (Product # MA5-32357) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:50-1:100

Immunocytochemistry (ICC)

1:50-1:200

Immunofluorescence (IF)

1:50-1:200

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

1:1,000-1:2,000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

SD20-50

Immunogen

Full length protein

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 1% BSA, 40% Glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

A family of proteins designated as Cdk's are critical regulators of cell cycle progression. The prototype member of this family, p34 / Cdc 2, and a related protein Cdk 2, function late in the cycle while Cdk 4 and Cdk 6 are critically involved in G1 to S progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.