Invitrogen

CDK7 Monoclonal Antibody (31TF2-1F8)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

26 References

View all (26) CDK7 antibodies

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Datasheet

Protocols

Questions & Answers

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CDK7 Antibody in Western Blot (WB) — Western blot was performed using Anti-CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001) and a 39 kDa band corresponding to CDK7 was observed across cell lines and tissue extracts tested along with a 25kDa band which corresponds to free IgG. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), HeLa (Lane 4), Jurkat (Lane 5), HCT116 (Lane 6), K-562 (Lane 7), tissue extracts of Mouse Heart (Lane 8), Mouse Testis (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution), 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CDK7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with CDK7 siRNA and decrease in signal intensity was observed in western blot application using CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001). {KD}

Product Details

MA3-001

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:2,000	View 23 publications 23 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Non-human primate, Pig, Yeast
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	31TF2-1F8
Immunogen	Synthetic peptide MALDVKSRAKRYEKLDFLGEGQ corresponding to aa 1-22 of human CDK7 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	ascites
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2608624

Product Specific Information

MA3-001 has been used successfully in Western blotting to detect Cyclin dependent kinase 7 in human lysates.

Target Information

The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Response of Two Mytilids to a Heatwave: The Complex Interplay of Physiology, Behaviour and Ecological Interactions.

PloS one

Olabarria C,Gestoso I,Lima FP,Vázquez E,Comeau LA,Gomes F,Seabra R,Babarro JM

MA3-001 was used in western blot to study how species interactions regulate responses to environmental stress

Fri May 19 00:00:00 EDT 2017

Effects of the Bacterial Extract OM-85 on Phagocyte Functions and the Stress Response.

Mediators of inflammation

Baladi S,Kantengwa S,Donati YR,Polla BS

MA3-001 was used in Western Blotting to establish that the bacterial extract OM-85 is able to modulate metabolic functions in monocytes.

Mon Sep 10 00:00:00 EDT 2012

Sublethal stress: Impact of solar UV radiation on protein synthesis in the copepod Acartia tonsa.

Journal of experimental marine biology and ecology

Tartarotti B,Torres JJ

MA3001 was used in western blot to study the induction of heat shock proteins in the marine copepod Acartia tonsa after UV exposure

Wed Jul 15 00:00:00 EDT 2009

Effects of neurotoxic insecticides on heat-shock proteins and cytokine transcription in Chinook salmon (Oncorhynchus tshawytscha).

Ecotoxicology and environmental safety

Eder KJ,Leutenegger CM,Köhler HR,Werner I

MA3001 was used in western blot to describe the effects of chlorpyrifos and esfenvalerate on juvenile Chinook salmon

Thu Jan 01 00:00:00 EST 2009

The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae.

Cellular & molecular biology letters

Zhang H,Hu B,Ji Y,Kato A,Song Y

MA3-001 was used in western blot to explore the effect of calnexin deletion on BiP

Mon Dec 29 00:00:00 EST 2008

Effects of environmental stress on intertidal mussels and their sea star predators.

Oecologia

Petes LE,Mouchka ME,Milston-Clements RH,Momoda TS,Menge BA

MA3-001 was used in western blot to study the effects of environmental stress on intertidal mussels and their sea star predators

Sun Jun 01 00:00:00 EDT 2008

An inducible 70 kDa-class heat shock protein is constitutively expressed during early development and diapause in the annual killifish Austrofundulus limnaeus.

Cell stress & chaperones

Podrabsky JE,Somero GN

MA3001 was used in western blot to study heat shock protein 70 levels in Austrofundulus limnaeus

Thu Oct 18 00:00:00 EDT 2007

Pesticide and pathogen: heat shock protein expression and acetylcholinesterase inhibition in juvenile Chinook salmon in response to multiple stressors.

Environmental toxicology and chemistry

Eder KJ,Köhler HR,Werner I

MA3001 was used in western blot to compare heat shock protein induction in salmon following exposure to individual pesticides, virus, or both stressors

Fri Jun 01 00:00:00 EDT 2007

Comparisons of stress proteins and soluble carbohydrate in encysted embryos of Artemia franciscana and two species of Parartemia.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Clegg JS,Campagna V

MA3-001 was used in western blot to compare stress proteins and alcohol-soluble carbohydrates present in the cysts of brine shrimp

Sun Oct 01 00:00:00 EDT 2006

Temperature differentially affects adenosine triphosphatase activity in Hsc70 orthologs from Antarctic and New Zealand notothenioid fishes.

Cell stress & chaperones

Place SP,Hofmann GE

MA3001 was used in western blot to study the effect of temperature on Hsc70 adenosine triphosphatase activity in Trematomus bernacchii and Notothenia angustata

Tue May 30 00:00:00 EDT 2006

Turning up the heat: the effects of thermal acclimation on the kinetics of hsp70 gene expression in the eurythermal goby, Gillichthys mirabilis.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Lund SG,Ruberté MR,Hofmann GE

MA3-001 was used in western blot to examine the plasticity of the heat shock protein response in ectothermic organisms

Sat Apr 01 00:00:00 EST 2006

Mytilus trossulus hsp70 as a biomarker for arsenic exposure in the marine environment: laboratory and real-world results.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals

La Porte PF

MA3001 was used in western blot to study Mytilus trossulus hsp70 as a biomarker of marine environmental arsenic exposure

Tue Jan 31 00:00:00 EST 2006

Disruption of the endoplasmic reticulum by cytotoxins in LLC-PK1 cells.

Toxicology letters

Ryan PM,Bedard K,Breining T,Cribb AE

MA3001 was used in western blot to test if the endoplasmic reticulum is disrupted by iodoacetamide, tert-butylhydroperoxide, or sulfamethoxazole hydroxylamine

Tue Nov 15 00:00:00 EST 2005

Desiccation tolerance in encysted embryos of the animal extremophile, artemia.

Integrative and comparative biology

Clegg JS

MA3-001 was used in western blot to discuss features of encysted embryos formed by Artemia franciscana

Tue Nov 01 00:00:00 EST 2005

Sublethal toxicity of orchard stormwater runoff in Sacramento splittail (Pogonichthys macrolepidotus) larvae.

Marine environmental research

Teh SJ,Deng D,Werner I,Teh F,Hung SS

MA3001 was used in western blot to examine the effects of storm water runoff from sections of a plum orchard treated with esfenvalerate or diazinon using Pogonichthys macrolepidotus larvae

Fri Apr 01 00:00:00 EST 2005

Magnitude and duration of thermal stress determine kinetics of hsp gene regulation in the goby Gillichthys mirabilis.

Physiological and biochemical zoology : PBZ

Buckley BA,Hofmann GE

MA3-001 was used in western blot to report that both the magnitude and the duration of a heat shock determine the intensity of heat shock gene induction in Gillichthys mirabilis

Tue Dec 21 00:00:00 EST 2004

Habitat diversity and adaptation to environmental stress in encysted embryos of the crustacean Artemia.

Journal of biosciences

Tanguay JA,Reyes RC,Clegg JS

MA3-001 was used in western blot to discuss thermal tolerance and stress protein levels in brine shrimp embryos

Wed Dec 01 00:00:00 EST 2004

The influence of salinity on the heat-shock protein response of Potamocorbula amurensis (Bivalvia).

Marine environmental research

Werner I

MA3-001 was used in western blot to study the effect of salinity on the heat-shock protein response of Potamocorbula amurensis

Mon Aug 30 00:00:00 EDT 2004

Molecular and cellular biomarker responses to pesticide exposure in juvenile chinook salmon (Oncorhynchus tshawytscha).

Marine environmental research

Eder KJ,Leutenegger CM,Wilson BW,Werner I

MA3-001 was used in western blot to evaluate the effects of chlorpyrifos and esfenvalerate on juvenile Chinook salmon

Mon Aug 30 00:00:00 EDT 2004

Biomarker responses in Macoma nasuta (Bivalvia) exposed to sediments from northern San Francisco Bay.

Marine environmental research

Werner I,Teh SJ,Datta S,Lu X,Young TM

MA3-001 was used in western blot to assess biomarker responses and survival of Macoma nasuta exposed to sediments collected from six locations in northern San Francisco Bay

Mon Aug 30 00:00:00 EDT 2004

Heat-shock protein 70 (Hsp70) as a biochemical stress indicator: an experimental field test in two congeneric intertidal gastropods (genus: Tegula).

The Biological bulletin

Tomanek L,Sanford E

MA3001 was used in western blot to study the role of Hsp70 as a biochemical stress indicator in two congeneric intertidal gastropods

Mon Dec 01 00:00:00 EST 2003

Effects of dietary exposure to the pyrethroid pesticide esfenvalerate on medaka (Oryzias latipes).

Marine environmental research

Werner I,Geist J,Okihiro M,Rosenkranz P,Hinton DE

MA3-001 was used in western blot to test if dietary esfenvalerate is toxic to medaka

Tue Nov 19 00:00:00 EST 2002

Heat shock cognate protein 70 is involved in rotavirus cell entry.

Journal of virology

Guerrero CA,Bouyssounade D,Zárate S,Isa P,López T,Espinosa R,Romero P,Méndez E,López S,Arias CF

MA3-001 was used in immunocytochemistry to report that heat shock cognate protein is a receptor for rotaviruses

Mon Apr 01 00:00:00 EST 2002

Small heat shock protein p26 associates with nuclear lamins and HSP70 in nuclei and nuclear matrix fractions from stressed cells.

Journal of cellular biochemistry

Willsie JK,Clegg JS

MA3001 was used in western blot to determine the roles of p26 and other heat shock proteins in stabilizing nuclear matrix proteins

Tue Mar 19 00:00:00 EST 2002

Heat-shock protein expression is absent in the antarctic fish Trematomus bernacchii (family Nototheniidae).

The Journal of experimental biology

Hofmann GE,Buckley BA,Airaksinen S,Keen JE,Somero GN

MA3-001 was used in western blot to report the absence of a heat-shock response in Trematomus bernacchii

Tue Aug 01 00:00:00 EDT 2000

Expression of 70 kDa heat shock proteins in antarctic and New Zealand notothenioid fish.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Carpenter CM,Hofmann GE

MA3-001 was used in western blot to demonstrate constitutive expression of some members of the 70 kDa Hsp multigene family in Antarctic and cold temperate notothenioid fishes

Tue Feb 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: 39 KDa protein kinase; CAK; CDK; CDK-activating kinase; CDK-activating kinase 1; Cell division protein kinase 7; CR4 protein kinase; CRK4; CRK4 PK (CDC2-related-kinase-4 protein kinase); Cyclin-dependent kinase 7; cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase); cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); EC 2.7.11.22; EC 2.7.11.23; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; OTTHUMP00000222032; OTTHUMP00000222033; P39 Mo15; Protein-tyrosine kinase MPK-7; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subunit

Gene Aliases: AI323415; AI528512; C230069N13; CAK; CAK1; CDK7; CDKN7; Crk4; HCAK; MO15; Mpk-7; p39MO15; STK1

UniProt ID: (Human) P50613, (Mouse) Q03147

Entrez Gene ID: (Human) 1022, (Mouse) 12572

Molecular Function: non-receptor serine/threonine protein kinase