Invitrogen

CDK7 Monoclonal Antibody (31TF2-1F8)

FIGURE: 1 / 5

CDK7 Antibody (MA3-001) in WB

Western blot was performed using Anti-CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001) and a 39 kDa band corresponding to CDK7 was observed across cell lines and tissue extracts tested along with a 25kDa band which corresponds to free IgG. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), Hep G2 (Lane 2), MCF-7 (Lane 3), HeLa (Lane 4), Jurkat (Lane 5), HCT116 (Lane 6), K-562 (Lane 7), tissue extracts of Mouse Heart (Lane 8), Mouse Testis (Lane 9) and Mouse Ovary (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protei... View More

Product Image Gallery

CDK7 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with CDK7 siRNA and decrease in signal intensity was observed in western blot application using CDK7 Monoclonal Antibody (31TF2-1F8) (Product # MA3-001). {KD}

Product Details

MA3-001

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 24 publications 24 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Fish, Human, Non-human primate, Pig, Yeast

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

31TF2-1F8

Immunogen

Synthetic peptide MALDVKSRAKRYEKLDFLGEGQ corresponding to aa 1-22 of human CDK7

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2608624

Product Specific Information

MA3-001 has been used successfully in Western blotting to detect Cyclin dependent kinase 7 in human lysates.

Target Information

The trimeric complex of CDK7, cyclin H and MNAT1, functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This complex is thought to serve as a direct link between the regulation of transcription and the cell cycle. The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). CDK7 may be a target of germline cancer-inducing mutations.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Response of Two Mytilids to a Heatwave: The Complex Interplay of Physiology, Behaviour and Ecological Interactions.

PloS one

Olabarria C,Gestoso I,Lima FP,Vázquez E,Comeau LA,Gomes F,Seabra R,Babarro JM

MA3-001 was used in western blot to study how species interactions regulate responses to environmental stress

Fri May 19 00:00:00 UTC 2017

Effects of neurotoxic insecticides on heat-shock proteins and cytokine transcription in Chinook salmon (Oncorhynchus tshawytscha).

Ecotoxicology and environmental safety

Eder KJ,Leutenegger CM,Köhler HR,Werner I

MA3001 was used in western blot to describe the effects of chlorpyrifos and esfenvalerate on juvenile Chinook salmon

Thu Jan 01 00:00:00 UTC 2009

Sublethal stress: Impact of solar UV radiation on protein synthesis in the copepod Acartia tonsa.

Journal of experimental marine biology and ecology

Tartarotti B,Torres JJ

MA3001 was used in western blot to study the induction of heat shock proteins in the marine copepod Acartia tonsa after UV exposure

Wed Jul 15 00:00:00 UTC 2009

The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae.

Cellular & molecular biology letters

Zhang H,Hu B,Ji Y,Kato A,Song Y

MA3-001 was used in western blot to explore the effect of calnexin deletion on BiP

Mon Dec 29 00:00:00 UTC 2008

Effects of environmental stress on intertidal mussels and their sea star predators.

Oecologia

Petes LE,Mouchka ME,Milston-Clements RH,Momoda TS,Menge BA

MA3-001 was used in western blot to study the effects of environmental stress on intertidal mussels and their sea star predators

Sun Jun 01 00:00:00 UTC 2008

Pesticide and pathogen: heat shock protein expression and acetylcholinesterase inhibition in juvenile Chinook salmon in response to multiple stressors.

Environmental toxicology and chemistry

Eder KJ,Köhler HR,Werner I

MA3001 was used in western blot to compare heat shock protein induction in salmon following exposure to individual pesticides, virus, or both stressors

Fri Jun 01 00:00:00 UTC 2007

An inducible 70 kDa-class heat shock protein is constitutively expressed during early development and diapause in the annual killifish Austrofundulus limnaeus.

Cell stress & chaperones

Podrabsky JE,Somero GN

Published figure using CDK7 monoclonal antibody (Product # MA3-001) in Western Blot

Thu Oct 18 00:00:00 UTC 2007

Turning up the heat: the effects of thermal acclimation on the kinetics of hsp70 gene expression in the eurythermal goby, Gillichthys mirabilis.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Lund SG,Ruberté MR,Hofmann GE

MA3-001 was used in western blot to examine the plasticity of the heat shock protein response in ectothermic organisms

Sat Apr 01 00:00:00 UTC 2006

Comparisons of stress proteins and soluble carbohydrate in encysted embryos of Artemia franciscana and two species of Parartemia.

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology

Clegg JS,Campagna V

MA3-001 was used in western blot to compare stress proteins and alcohol-soluble carbohydrates present in the cysts of brine shrimp

Sun Oct 01 00:00:00 UTC 2006

Disruption of the endoplasmic reticulum by cytotoxins in LLC-PK1 cells.

Toxicology letters

Ryan PM,Bedard K,Breining T,Cribb AE

MA3001 was used in western blot to test if the endoplasmic reticulum is disrupted by iodoacetamide, tert-butylhydroperoxide, or sulfamethoxazole hydroxylamine

Tue Nov 15 00:00:00 UTC 2005

Temperature differentially affects adenosine triphosphatase activity in Hsc70 orthologs from Antarctic and New Zealand notothenioid fishes.

Cell stress & chaperones

Place SP,Hofmann GE

MA3001 was used in western blot to study the effect of temperature on Hsc70 adenosine triphosphatase activity in Trematomus bernacchii and Notothenia angustata

Tue May 30 00:00:00 UTC 2006

Sublethal toxicity of orchard stormwater runoff in Sacramento splittail (Pogonichthys macrolepidotus) larvae.

Marine environmental research

Teh SJ,Deng D,Werner I,Teh F,Hung SS

MA3001 was used in western blot to examine the effects of storm water runoff from sections of a plum orchard treated with esfenvalerate or diazinon using Pogonichthys macrolepidotus larvae

Fri Apr 01 00:00:00 UTC 2005

Desiccation tolerance in encysted embryos of the animal extremophile, artemia.

Integrative and comparative biology

Clegg JS

MA3-001 was used in western blot to discuss features of encysted embryos formed by Artemia franciscana

Tue Nov 01 00:00:00 UTC 2005

Comparison of Hsc70 orthologs from polar and temperate notothenioid fishes: differences in prevention of aggregation and refolding of denatured proteins.

American journal of physiology. Regulatory, integrative and comparative physiology

Place SP,Hofmann GE

MA3-001 was used in Western Blot to indicate the function of Hsc70 in notothenioid fishes maps onto their thermal history and that temperature selection has acted on these molecular chaperones.

Sun May 01 00:00:00 UTC 2005

Mytilus trossulus hsp70 as a biomarker for arsenic exposure in the marine environment: laboratory and real-world results.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals

La Porte PF

MA3001 was used in western blot to study Mytilus trossulus hsp70 as a biomarker of marine environmental arsenic exposure

Tue Jan 31 00:00:00 UTC 2006

The influence of salinity on the heat-shock protein response of Potamocorbula amurensis (Bivalvia).

Marine environmental research

Werner I

MA3-001 was used in western blot to study the effect of salinity on the heat-shock protein response of Potamocorbula amurensis

Mon Aug 30 00:00:00 UTC 2004

Molecular and cellular biomarker responses to pesticide exposure in juvenile chinook salmon (Oncorhynchus tshawytscha).

Marine environmental research

Eder KJ,Leutenegger CM,Wilson BW,Werner I

MA3-001 was used in western blot to evaluate the effects of chlorpyrifos and esfenvalerate on juvenile Chinook salmon

Mon Aug 30 00:00:00 UTC 2004

Biomarker responses in Macoma nasuta (Bivalvia) exposed to sediments from northern San Francisco Bay.

Marine environmental research

Werner I,Teh SJ,Datta S,Lu X,Young TM

MA3-001 was used in western blot to assess biomarker responses and survival of Macoma nasuta exposed to sediments collected from six locations in northern San Francisco Bay

Mon Aug 30 00:00:00 UTC 2004

Habitat diversity and adaptation to environmental stress in encysted embryos of the crustacean Artemia.

Journal of biosciences

Tanguay JA,Reyes RC,Clegg JS

MA3-001 was used in western blot to discuss thermal tolerance and stress protein levels in brine shrimp embryos

Wed Dec 01 00:00:00 UTC 2004

Magnitude and duration of thermal stress determine kinetics of hsp gene regulation in the goby Gillichthys mirabilis.

Physiological and biochemical zoology : PBZ

Buckley BA,Hofmann GE

MA3-001 was used in western blot to report that both the magnitude and the duration of a heat shock determine the intensity of heat shock gene induction in Gillichthys mirabilis

Tue Dec 21 00:00:00 UTC 2004

Heat-shock protein 70 (Hsp70) as a biochemical stress indicator: an experimental field test in two congeneric intertidal gastropods (genus: Tegula).

The Biological bulletin

Tomanek L,Sanford E

MA3001 was used in western blot to study the role of Hsp70 as a biochemical stress indicator in two congeneric intertidal gastropods

Mon Dec 01 00:00:00 UTC 2003

Heat shock cognate protein 70 is involved in rotavirus cell entry.

Journal of virology

Guerrero CA,Bouyssounade D,Zárate S,Isa P,López T,Espinosa R,Romero P,Méndez E,López S,Arias CF

MA3-001 was used in immunocytochemistry to report that heat shock cognate protein is a receptor for rotaviruses

Mon Apr 01 00:00:00 UTC 2002

Small heat shock protein p26 associates with nuclear lamins and HSP70 in nuclei and nuclear matrix fractions from stressed cells.

Journal of cellular biochemistry

Willsie JK,Clegg JS

MA3001 was used in western blot to determine the roles of p26 and other heat shock proteins in stabilizing nuclear matrix proteins

Tue Mar 19 00:00:00 UTC 2002

Effects of dietary exposure to the pyrethroid pesticide esfenvalerate on medaka (Oryzias latipes).

Marine environmental research

Werner I,Geist J,Okihiro M,Rosenkranz P,Hinton DE

MA3-001 was used in western blot to test if dietary esfenvalerate is toxic to medaka

Tue Nov 19 00:00:00 UTC 2002

Heat-shock protein expression is absent in the antarctic fish Trematomus bernacchii (family Nototheniidae).

The Journal of experimental biology

Hofmann GE,Buckley BA,Airaksinen S,Keen JE,Somero GN

MA3-001 was used in western blot to report the absence of a heat-shock response in Trematomus bernacchii

Tue Aug 01 00:00:00 UTC 2000

Expression of 70 kDa heat shock proteins in antarctic and New Zealand notothenioid fish.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

Carpenter CM,Hofmann GE

MA3-001 was used in western blot to demonstrate constitutive expression of some members of the 70 kDa Hsp multigene family in Antarctic and cold temperate notothenioid fishes

Tue Feb 01 00:00:00 UTC 2000

Effects of the Bacterial Extract OM-85 on Phagocyte Functions and the Stress Response.

Mediators of inflammation

Baladi S,Kantengwa S,Donati YR,Polla BS

MA3-001 was used in Western Blotting to establish that the bacterial extract OM-85 is able to modulate metabolic functions in monocytes.

Mon Sep 10 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: 39 KDa protein kinase; CAK; CDK; CDK-activating kinase; CDK-activating kinase 1; Cell division protein kinase 7; CR4 protein kinase; CRK4; CRK4 PK (CDC2-related-kinase-4 protein kinase); Cyclin-dependent kinase 7; cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase); cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); EC 2.7.11.22; EC 2.7.11.23; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; OTTHUMP00000222032; OTTHUMP00000222033; P39 Mo15; Protein-tyrosine kinase MPK-7; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subunit

View more View less

Gene Aliases: AI323415; AI528512; C230069N13; CAK; CAK1; CDK7; CDKN7; Crk4; HCAK; MO15; Mpk-7; p39MO15; STK1

View more View less

UniProt ID: (Human) P50613, (Mouse) Q03147

View more View less

Entrez Gene ID: (Human) 1022, (Mouse) 12572

View more View less

Function(s)

transcription coactivator activity protein kinase activity protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding protein C-terminus binding DNA-dependent ATPase activity RNA polymerase II carboxy-terminal domain kinase activity kinase activity androgen receptor binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding non-receptor serine/threonine protein kinase

Process(es)

regulation of cyclin-dependent protein serine/threonine kinase activity G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping protein phosphorylation cell cycle arrest cell proliferation androgen receptor signaling pathway snRNA transcription from RNA polymerase II promoter positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell division DNA repair transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus cell cycle phosphorylation regulation of cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CDK7 Monoclonal Antibody (31TF2-1F8)

CDK7 Antibody (MA3-001) in WB

Product Details

MA3-001

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!