|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human protein purified from E.coli(His/ABD-CDK7 136-346 a.a.)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg|
|Western Blot (WB)||0.2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is HeLa cells.
Cyclin-dependent kinase 7 (CDK7) has essential roles in the cell-division cycle as a CDK-activating kinase (CAK) and in the transcription as a component of the general transcription factor TFIIH. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK), phosphorylating cell-cycle CDKs. It is also an essential component of the transcription factor TFIIH, which phosphorylates the C-terminal domain (CTD) of the largest subunit of Pol II. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. Embryonic stem cells (ESCs) shows a very unusual cell cycle structure, characterized by a short G1 phase and a high proportion of cells in S-phase. This is associated with a unique mechanism of cell cycle regulation by the activity of cyclin dependent protein kinase (Cdk). The unique cell cycle structure and mechanism of cell cycle control indicates that the cell cycle machinery plays a role in establishment or maintenance of the stem cell state.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.