|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||His-tagged recombinant full-length mouse p27|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In immunoprecipitation applications, CDK4 has been found to co-precipitate.
The p27 kip1 functions as a negative regulator of G1 mouse expression and has been proposed to function as a possible mediator of TGFb induced G1 arrest.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SHIP2 signaling in normal and pathological situations: Its impact on cell proliferation.
MA1-12302 was used in western blot to study the roles of SHIP2 signaling in normal and tumor cells
|Elong Edimo W,Schurmans S,Roger PP,Erneux C||Advances in biological regulation (54:142)||2014|
CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point.
MA1-12302 was used in western blot to study the roles CDK7 and p21 in regulating the T172 phosphorylation of CDK4 at the restriction point
|Bisteau X,Paternot S,Colleoni B,Ecker K,Coulonval K,De Groote P,Declercq W,Hengst L,Roger PP||PLoS genetics (9:null)||2013|
Expression of p8 protein in breast carcinoma; an inverse relationship with apoptosis.
MA1-12302 was used in immunohistochemistry to study the expression of p8 protein in breast carcinoma and its relationship with apoptosis
|Ito Y,Yoshida H,Motoo Y,Iovanna JL,Nakamura Y,Kakudo K,Uruno T,Takamura Y,Miya A,Noguchi S,Kuma K,Miyauchi A||Anticancer research (25:833)||2005|
Expression of the components of the Cip/Kip family in malignant lymphoma of the thyroid.
MA1-12302 was used in immunohistochemistry to study the expression of components of the Cip/Kip family in malignant lymphoma of the thyroid
|Ito Y,Yoshida H,Matsuzuka F,Matsuura N,Nakamura Y,Nakamine H,Kakudo K,Kuma K,Miyauchi A||Pathobiology : journal of immunopathology, molecular and cellular biology (71:164)||2004|