Immunofluorescent detection of p19Arf in wild type mouse embryo fibroblasts (MEF) at passage 5 (lanes 1, 2) and NIH3T3 cells, which have deleted the Arf gene (lanes 3, 4) were fixed in methanol/acetone and probed with MA1-16664, followed by a fluorescently labeled anti-rat IgG secondary antibody (lanes 1, 3). Cells were also stained with Hoechst dye to reveal the position of nuclei (lanes 2, 4).
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rat / IgG2b|
|Immunogen||Synthetic peptide containing amino acids within 1-100 of the murine p19ARF.|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with human or Golden Syrian hamster.
In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various onocoproteins via the p53 regulatory loop. These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Loss of Keratin 17 induces tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo.
MA1-16664 was used in immunohistochemistry and western blot to analyze the induction of tissue-specific cytokine polarization and cellular differentiation in HPV16-driven cervical tumorigenesis in vivo due to loss of keratin 17
|Hobbs RP,Batazzi AS,Han MC,Coulombe PA||Oncogene (35:5653)||2016|
Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport.
MA1-16664 was used in western blot to investigate the distribution and function of SMAP2 in trans-Golgi network
|Funaki T,Kon S,Ronn RE,Henmi Y,Kobayashi Y,Watanabe T,Nakayama K,Tanabe K,Satake M||Cell structure and function (36:83)||2011|