|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:500-1:2500|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide to human p14ARF (C-terminal sequence).|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Suggested positive control: BT549 or HeLa whole cell extracts, antigen standard for CDKN2A (transient overexpression lysate).
The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.
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Protein Aliases: Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4 inhibitor p16INK4; CDK4I; CDKN2; CDKN2A; Cell cycle negative regulator beta; CMM2; Cyclin dependent ki; Cyclin dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); MTS-1; Multiple tumor suppressor 1; p14ARF; p16-INK4; p16-INK4a; Tumor suppressor ARF
Gene Aliases: ARF; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16
UniProt ID: (Human) P42771
Entrez Gene ID: (Human) 1029
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