|ChIP assay (ChIP)||Assay-dependent|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:500-1:1500|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH, within amino acids 54-75 of Mouse p19ARF.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris, pH 7.6|
|Contains||0.1% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-30670 detects CDKN2A from human and mouse samples.
PA1-30670 has been successfully used in ELISA, ChIP, immunofluorescence, immunohistochemistry (paraffin tissue), Western blot, immunoprecipitation applications.
The PA1-30670 immunogen is: Synthetic peptide conjugated to KLH, within amino acids 54-75 of Mouse p19ARF.
The four known 15-to 19 kDa INK4 proteins (p15INK4b, p16INK4a, p18INK4c andp19INK4d) bind and inhibit CDK4 and CDK6 but not other CDKs, including cyclin E-Cdk2, cyclin A-Cdk2 and cyclin B-Cdk1. Like the three D-type cyclins, the INK4 genes are expressed i
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Alternative reading frame; ARF; CDK4 inhibitor p16-INK4; CDK4I; cdkn2a; cell cycle negative regulator beta; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; mitochondrial smARF; MTS-1; MTS1; Multiple tumor suppressor 1; p14ARF; p16-INK4; p16-INK4a; p16INK4c; p19ARF; Tumor suppressor ARF
Gene Aliases: ARF; ARF-INK4a; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; INK4a-ARF; Ink4a/Arf; MLM; MTS-1; MTS1; P14; P14ARF; P16; p16(INK4a); P16-INK4A; P16INK4; P16INK4A; P19; p19<ARF>; P19ARF; Pctr1; TP16
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