Immunofluorescent analysis of CDX2 (green) showing staining in the nucleus of SW480 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CDX2 monoclonal antibody (Product # MA5-14494) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to residues near the N-terminus of human CDX2|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA5-14494 targets CDX2 in WB, IHC (P), ICC/IF and FACS applications and shows reactivity with Human and Mouse samples.
The MA5-14494 immunogen is a synthetic peptide corresponding to residues near the N-terminus of human CDX2.
The caudal-related homeodomain protein2 (CDX2) is a transcription factor shown to play a role in the development of small and large intestine in mammals and in the differentiation of intestinal epithelial cells. It has been shown that CDX2 protein detection by IHC coorelates with RNA transcript levels. In studies using 745 cancers from many anatomic sites, colonic adenocarcinomas demonstrated strong staining in 90% of the cases, while adenocarcinomas of the stomach, esophagus and ovary showed extensive staining in only 30% of the cases. Stains of CDX2 and Villin are useful markers in differntial diagnosis of bladder adenocarcinoma and secondary colorectal adenocarcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The relationship among PDX1, CDX2, and mucin profiles in gastric carcinomas; correlations with clinicopathologic parameters.
MA5-14494 was used in immunohistochemistry to study the association of PDX1 expression with various clinicopathologic parameters in gastric carcinomas
|Oz Puyan F,Can N,Ozyilmaz F,Usta U,Sut N,Tastekin E,Altaner S||Journal of cancer research and clinical oncology (137:1749)||2011|