Immunofluorescent analysis of CDw75 (green) showing staining in the membrane and cytoplasm of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CDw75 monoclonal antibody (Product # MA5-11900) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgM, kappa|
|Immunogen||Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
|Western Blot (WB)||1:100-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 3 publications below|
MA5-11900 targets CDw75 in FACS, IF, WB and IHC (P) applications and shows reactivity with Human and Mouse samples. Antibody does not react with rat. The molecular weight of the antigen is 53 and 87 kDa.
The MA5-11900 immunogen is nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
CDw75 is a neuraminidase-sensitive sialoprotein, present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. LN1 reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin's disease. LN1 is shown to be a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Propagation of oestrogen receptor-positive and oestrogen-responsive normal human breast cells in culture.
MA5-11900 was used in immunohistochemistry to analyze propagation of oestrogen-responsive and oestrogen receptor-positive normal human breast cells in culture
|Fridriksdottir AJ,Kim J,Villadsen R,Klitgaard MC,Hopkinson BM,Petersen OW,Rønnov-Jessen L||Nature communications (6:null)||2015|
Antibodies are forever: a study using 12-26-year-old expired antibodies.
MA5-11900 was used in immunohistochemistry to study the effective shelf-life of diagnostic antibodies
|Argentieri MC,Pilla D,Vanzati A,Lonardi S,Facchetti F,Doglioni C,Parravicini C,Cattoretti G||Histopathology (63:869)||2013|
Synthesis and expression of CDw75 antigen in human colorectal cancer.
MA5-11900 was used in immunohistochemistry to study the expression of the CDw75 antigen in human colorectal cancer
|Costa-Nogueira C,Villar-Portela S,Cuevas E,Gil-Martín E,Fernández-Briera A||BMC cancer (9:null)||2009|