|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||5-20µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Radioimmune Assays (RIA)||Assay Dependent|
|Western Blot (WB)||1µg/ml|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified CEA from pooled tumors|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
The predicted MW of CEA is ~77kD, but by Western blot MIC0101 detects CEA with varying degrees of glycosylation at ~77-180kD.
MIC0101 was formerly sold as a Seradyn product.
This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Carcinoembryonic Antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CEA; Meconium antigen 100
Gene Aliases: CD66e; CEA; CEACAM5
UniProt ID: (Human) P06731
Entrez Gene ID: (Human) 1048
Molecular Function: G-protein coupled receptor cell adhesion molecule cytokine receptor defense/immunity protein hydrolase immunoglobulin receptor superfamily immunoglobulin superfamily cell adhesion molecule phosphatase protein phosphatase receptor
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