|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.2 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||1.2 ug/10^6 cells|
|Western Blot (WB)||4 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes native human CEACAM5 protein expressed in transfected mammalian cells.
CEACAM5 (CEA-related cell adhesion molecule 5, CEA) belongs to the carcinoembryonic antigen (CEA) gene family. It encodes a glycosyl phosphatidyl inositol (GPI)-linked glycoprotein with a MW of 180-200kDa which is most strongly expressed on epithelial cells of the fetal and adult colon and to a minor extent on epithelial cells of the stomach and sweat glands, squamous epithelial cell of the tongue, esophagus and cervix. CEACAM5 is used as a tumor marker for early detection of recurrent disease due to its expression in adenocarcinomas of the colon, lung, breast, stomach and pancreas and in mucinous ovarian carcinomas. Like all members of the CEACAM family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by six immunoglobulin constant-like A (A1, A2, A3) and B domains (B1, B2, B3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.