Immunofluorescent analysis of CD66e (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD66e monoclonal antibody (Product # MA5-13714) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Human colon carcinoma extract|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1000|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Flow Cytometry (Flow)||See 2 publications below|
|Immunohistochemistry (IHC)||See 9 publications below|
|ELISA (ELISA)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
MA5-13714 targets CD66e in ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.
The MA5-13714 immunogen is human colon carcinoma extract.
Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comparative expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1 in gastric and colorectal cancer.
MA5-13714 was used in immunohistochemistry - paraffin section and western blot to compare colorectal and gastric cancer and their expression patterns and diagnostic efficacies of SR splicing factors and HNRNPA1
|Park WC,Kim HR,Kang DB,Ryu JS,Choi KH,Lee GO,Yun KJ,Kim KY,Park R,Yoon KH,Cho JH,Lee YJ,Chae SC,Park MC,Park DS||BMC cancer (16:null)||2016|
|Not Applicable||Not Cited||
Sebaceous carcinoma of the eyelids: thirty cases from Japan.
MA5-13714 was used in immunohistochemistry - paraffin section to characterize thirty cases of sebaceous carcinoma of the eyelids
|Izumi M,Mukai K,Nagai T,Matsubayashi J,Iwaya K,Chiu CS,Goto H||Pathology international (58:483)||2008|
|Not Applicable||Not Cited||
Oral premalignant lesions induce immune reactivity to both premalignant oral lesions and head and neck squamous cell carcinoma.
MA5-13714 was used in immunohistochemistry - paraffin section to study premalignant lesions for markers of head and neck squamous cell carcinoma
|Young MR,Neville BW,Chi AC,Lathers DM,Boyd Gillespie M,Day TA||Cancer immunology, immunotherapy : CII (56:1077)||2007|
|Not Applicable||Not Cited||
Heightened susceptibility to chronic gastritis, hyperplasia and metaplasia in Kcnq1 mutant mice.
MA5-13714 was used in immunohistochemistry - paraffin section to use Kcnq1 mutant mice to study factor that promote gastric cancer
|Elso CM,Lu X,Culiat CT,Rutledge JC,Cacheiro NL,Generoso WM,Stubbs LJ||Human molecular genetics (13:2813)||2004|
Solid-pseudopapillary neoplasm of the pancreas: Clinicopathologic and immunohistochemical analysis of nine cases.
MA5-13714 was used in immunohistochemistry (paraffin) to review cases and literature involving solid-pseudopapillary neoplasm of the pancreas.
|Ozguven BY,Tuncel D,Polat N,Sakiz D,Kabukcuoglu F,Koksal H,Kaya H||Indian journal of pathology and microbiology (58:292)||2015|
|Not Applicable||Not Cited||
Global analysis of neutrophil responses to Neisseria gonorrhoeae reveals a self-propagating inflammatory program.
MA5-13714 was used in flow cytometry to analyze Neisseria gonorrhoeae and neutrophil response that suggests a self-propagating inflammatory program
|Sintsova A,Sarantis H,Islam EA,Sun CX,Amin M,Chan CH,Stanners CP,Glogauer M,Gray-Owen SD||PLoS pathogens (10:null)||2014|
Multiple breast cancer cell-lines derived from a single tumor differ in their molecular characteristics and tumorigenic potential.
MA5-13714 was used in flow cytometry, immunohistochemistry, and western blot to study the different molecular phenotype and tumorigenicity of 5 breast cancer cell lines derived from a single patient
|Mosoyan G,Nagi C,Marukian S,Teixeira A,Simonian A,Resnick-Silverman L,DiFeo A,Johnston D,Reynolds SR,Roses DF,Mosoian A||PloS one (8:null)||2013|
Arginase II expressed in cancer-associated fibroblasts indicates tissue hypoxia and predicts poor outcome in patients with pancreatic cancer.
MA5-13714 was used in immunohistochemistry to study the value of cancer-associated fibroblast arginase II expression as both a marker of hypoxia and a prognostic indicator in pancreatic ductal carcinoma
|Ino Y,Yamazaki-Itoh R,Oguro S,Shimada K,Kosuge T,Zavada J,Kanai Y,Hiraoka N||PloS one (8:null)||2013|
Sinonasal tract mucoepidermoid carcinoma: a clinicopathologic and immunophenotypic study of 19 cases combined with a comprehensive review of the literature.
MA5-13714 was used in immunohistochemistry to characterize primary sinonasal tract mucoepidermoid carcinomas
|Wolfish EB,Nelson BL,Thompson LD||Head and neck pathology (6:191)||2012|
Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.
MA5-13714 was used in immunohistochemistry to investigate deciduoid features of malignant mesothelioma by histomorphological and immunohistochemical evaluation
|Ustun H,Astarci HM,Sungu N,Ozdemir A,Ekinci C||Diagnostic cytopathology (39:402)||2011|
Low-grade mucinous adenocarcinoma of the uterine corpus: a rare and deceptively bland form of endometrial carcinoma.
MA5-13714 was used in immunohistochemistry to characterize a rare form of endometrial carcinoma
|Fujiwara M,Longacre TA||The American journal of surgical pathology (35:537)||2011|
In vitro constitution and in vivo implantation of engineered skin constructs with sweat glands.
MA5-13714 was used in immunohistochemistry to investigate the integration of sweat glands into engineered skin constructs in vitro and in vivo in order to improve the quality of tissue regeneration
|Huang S,Xu Y,Wu C,Sha D,Fu X||Biomaterials (31:5520)||2010|
Antigen expression of human eccrine sweat glands.
MA5-13714 was used in immunohistochemistry to identify the phenotypic markers of human eccrine sweat glands
|Li HH,Zhou G,Fu XB,Zhang L||Journal of cutaneous pathology (36:318)||2009|
Detection of micrometastases and skip metastases with ex vivo sentinel node mapping in carcinoma of the colon and rectum.
MA5-13714 was used in immunohistochemistry to study micrometastases and skip metastases in carcinoma of the colon and rectum
|Yagci G,Unlu A,Kurt B,Can MF,Kaymakcioglu N,Cetiner S,Tufan T,Sen D||International journal of colorectal disease (22:167)||2007|
Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients.
MA5-13714 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver
|Yesim G,Gupse T,Zafer U,Ahmet A||Journal of hepato-biliary-pancreatic surgery (12:502)||2005|
MA5-13714 was used in immunohistochemistry to report on the histological and clinical features of secretory meningiomas
|Colako¿lu N,Demirta¿ E,Oktar N,Yüntem N,Islekel S,Ozdamar N||Journal of neuro-oncology (62:233)||2003|
A genetically engineered adenovirus vector targeted to CD40 mediates transduction of canine dendritic cells and promotes antigen-specific immune responses in vivo.
MA5-13714 was used in ELISA to study the immune response effects of a genetically engineered adenovirus vector targeted to canine dendritic cell CD40
|Thacker EE,Nakayama M,Smith BF,Bird RC,Muminova Z,Strong TV,Timares L,Korokhov N,O'Neill AM,de Gruijl TD,Glasgow JN,Tani K,Curiel DT||Vaccine (27:7116)||2009|
Function of CSE1L/CAS in the secretion of HT-29 human colorectal cells and its expression in human colon.
MA5-13714 was used in western blot to study the regulatory role of CSE1L/CAS in the secretion of human colorectal cells
|Tsao TY,Tsai CS,Tung JN,Chen SL,Yue CH,Liao CF,Wang CC,Jiang MC||Molecular and cellular biochemistry (327:163)||2009|
Evaluation of the value of frozen tissue section used as "gold standard" for immunohistochemistry.
MA5-13714 was used in immunohistochemistry - frozen section to evaluate different fixation and antigen retrieval methods for immmunohistochemical studies on frozen tissues
|Shi SR,Liu C,Pootrakul L,Tang L,Young A,Chen R,Cote RJ,Taylor CR||American journal of clinical pathology (129:358)||2008|