Immunofluorescent analysis of CD66e (green) showing staining in the cytoplasm and membrane of HepG2 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD66e polyclonal antibody (Product # PA5-16665) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human CEA isolated from hepatic metastasis of colon adenocarcinoma|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:1000|
|Immunoprecipitation (IP)||10 µl/mg lysate|
|Western Blot (WB)||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
PA5-16665 targets CD66e in IHC (P), IP, ICC/IF, FACS and WB applications and shows reactivity with Human and mouse samples.
The PA5-16665 immunogen is human CEA isolated from hepatic metastasis of colon adenocarcinoma.
Carcinoembryonic antigen (CEA), is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibody to CEA is reportedly useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
New diagnostic markers in salivary gland tumors.
PA5-16665 was used in immunohistochemistry to study the expresion of tumor antigens in order to identify novel diagnostic markers of parotid gland tumors
|Schneider S,Kloimstein P,Pammer J,Brannath W,Grasl MCh,Erovic BM||European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (271:1999)||2014|
Prognostic and predictive markers in medullary thyroid carcinoma.
PA5-16665 was used in immunohistochemistry to identify markers with progniostic and predictive value in patients with medullary thyroid cancer
|Erovic BM,Kim D,Cassol C,Goldstein DP,Irish JC,Asa SL,Mete O||Endocrine pathology (23:232)||2012|