|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CEA partially purified from a perchloric acid extract from liver metastases of colonic tumors.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.2 ug/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||1-2 ug/10^6 cells|
|Western Blot (WB)||4 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes native human CEACAM1, 5 protein expressed in transfected mammalian cells.
Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker CEACAM1 expression is down-regulated in many tumors indicating a tumor-suppressive function. The anti-tumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells. The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.