|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant rat C/EBP alpha|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA1-825 detects C/EBP alpha protein in mouse samples.
PA1-825 has successfully been used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 30 and 43 kDa protein representing C/EBP alpha in mouse 3T3-L1 cells. These polypeptides are derived from alternative translation initiation.
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Circadian clock disruption improves the efficacy of chemotherapy through p73-mediated apoptosis.
PA1-825 was used in immunoprecipitation, immunoprecipitation, western blot, and western blot to investigate the effect of p73 on chemotherapy through circadian clock and cell apoptosis
|Lee JH,Sancar A||Proceedings of the National Academy of Sciences of the United States of America (108:10668)||2011|