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|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant full length CENP-E protein (human) from SP2/0 cells|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-5758 detects CENP-E in human samples.
MA1-5758 has been successfully used in immunoprecipitation, immunocytochemistry, immunofluorescence and Western blot procedures.
The MA1-5758 immunogen is recombinant full length CENP-E protein (human).
Store at 4°C for 1 month or at -20°C for longer periods.
The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle. The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner. Members of the first class include CENP-A, CENP-B, and CENP-C. The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F. CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex. CENP-F also shows a cell-cycle distribution that is both temporally regulated and diverse in terms of the structural components it localizes to and therefore may play a role in several mitotic events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CENP-E; Centromere autoantigen E (312kD); Centromere protein E; centromere protein E, 312kDa; Centromere-associated protein E; kinesin family member 10; Kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61
CENP-E; CENPE; KIF10; MCPH13; PPP1R61