|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (QASLDSIREAVINSQ) corresponding to the internal amino acids of AGAP2|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.3-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AGAP2 (also known as PIKE-A), a putative oncogene, shows elevated expression in cancer cells compared to non-transformed cells and has been implicated as a regulator of cancer cell invasion into normal tissues. A contributor to brain, eye, breast and liver cancers, AGAP2 belongs to the centaurin gamma-like family with two ANK repeats, an Arf-GAP domain, a Miro domain and a PH domain. It is a GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. The Arf-GAP domain interacts with the G domain and may regulate its GTPase activity. GAP activity of AGAP2 is stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and, to a lesser extent, by phosphatidylinositol 3,4,5-trisphosphate (PIP3). Phosphatidic acid potentiates PIP2 stimulation. Several isoforms of AGAP2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.