Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 2
CETP Antibody (MA1-050) in ICC/IF
Immunofluorescent analysis of CETP in HepG2 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a CETP monoclonal antibody (Product # MA1-050) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). CETP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Product Details
MA1-050
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rabbit
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
ATM192
Immunogen
Synthetic peptide corresponding to residues C D(149) S G R V R T D A P D(159) of human CETP protein.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_325404
Product Specific Information
MA1-050 detects cholesteryl ester transfer protein (CETP) from human and rabbit tissues.
MA1-050 has been successfully used in Western blot, immunoprecipitation, IF and ELISA protocols. By Western blot, this antibody detects a doublet at ~70 kDa from human serum representing CETP. This antibody can also be used to immunoprecipitate CETP activity from serum samples using protein G.
The MA1-050 immunogen is a synthetic peptide corresponding to residues C D(149) S G R V R T D A P D(159) of human CETP protein.
Target Information
It has been proposed that high density lipoproteins (HDLs) function jointly with lecithin:cholesterol acyltransferase and CETP to facilitate cholesterol transport from tissues to the liver. This mechanism, referred to as reverse cholesterol transport, is physiologically important because it maintains systemic cholesterol levels. CETP is responsible for neutral lipid transfer activity in plasma in numerous species. Since CETP is able to accelerate specifically the exchange of lipid components between pro- and anti-atherogenic lipoprotein fractions, it may be a key determinant of the global atherogenicity of the plasma lipoprotein profile and arises as a possible target in atherosclerosis prevention. CETP has an important role in reverse cholesterol transport and shaping and affecting the composition of plasma lipoproteins. In general elevated levels of CETP have been associated with increased risk of coronary heart disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BPI fold containing family F; Cholesteryl ester transfer protein; cholesteryl ester transfer protein plasma; cholesteryl ester transfer protein, plasma; Lipid transfer protein I
Gene Aliases: BPIFF; CETP; HDLCQ10
UniProt ID: (Human) P11597
Entrez Gene ID: (Human) 1071, (Rabbit) 100327267
triglyceride metabolic process
lipid transport
receptor-mediated endocytosis
cholesterol metabolic process
negative regulation of macrophage derived foam cell differentiation
regulation of cholesterol efflux
phospholipid transport
cholesterol transport
triglyceride transport
very-low-density lipoprotein particle remodeling
low-density lipoprotein particle remodeling
high-density lipoprotein particle remodeling
lipoprotein metabolic process
cholesterol homeostasis
reverse cholesterol transport
phosphatidylcholine metabolic process
lipid homeostasis
phospholipid homeostasis
triglyceride homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support