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CFL2 Antibody (MA5-36233) in ICC/IF
Immunocytochemistry analysis of CFL2 in U20S cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in CFL2 monoclonal antibody (Product # MA5-36233) at a dilution of 2 µg/mL (overnight, 4°C), followed by DyLight 488 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.
Product Details
MA5-36233
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
8C13
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cofilin 2/CFL2 (121-153aa KDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2884067
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human U2OS whole cell, human HepG2 whole cell, human T-47D whole cell, human Raji whole cell, human placenta tissue, human A549 whole cell, rat heart tissue, rat liver tissue, rat kidney tissue, rat brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, mouse brain tissue, mouse NIH/3T3 whole cell. IHC: human lung cancer tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue. ICC/IF: U20S cell. Flow: A549 cell, SiHa cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: COF2; cofilin 2 (muscle); Cofilin, muscle isoform; Cofilin-2; HGNC:1875; nemaline myopathy type 7
Gene Aliases: CFL2; NEM7
UniProt ID: (Human) Q9Y281, (Mouse) P45591
Entrez Gene ID: (Human) 1073, (Rat) 366624, (Mouse) 12632
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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