Abnova

CFLAR Monoclonal Antibody (2F12)

Product Image Gallery

CFLAR Antibody in Western Blot (WB) — CFLAR monoclonal antibody (M01), clone 2F12. Western Blot analysis of CFLAR expression in K-562.

CFLAR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunoperoxidase of monoclonal antibody to CFLAR on formalin-fixed paraffin-embedded human kidney. Antibody concentration is 3 µg/mL.

CFLAR Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged CFLAR is approximately 0.3 ng/mL as a capture antibody.

CFLAR Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of monoclonal antibody to CFLAR on HeLa cell. Antibody concentration is 10 µg/mL.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

3 µg/mL

ELISA (ELISA)

0.3 ng/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2F12

Immunogen

CFLAR (AAH01602, 178 a.a. approximately 277 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: AGTSYRNVLQ AAIQKSLKDP SNNFRLHNGR SKEQRLKEQL GAQQEPVKKS IQESEAFLPQ SIPEERYKMK SKPLGICLII DCIGNETELL RDTFTSLGYE

Target Information

FLIP (CFLAR) is an apoptosis regulator protein which functions as a crucial link between cell survival and cell death pathways in mammalian cells. It acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: c-FLIP; CASH; CASP8 and FADD-like apoptosis regulator; CASP8 and FADD-like apoptosis regulator subunit p12; CASP8 and FADD-like apoptosis regulator subunit p43; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; Casper; Cellular FLICE-like inhibitory protein; CLARP; FADD-like antiapoptotic molecule 1; FLAME-1; I-FLICE; Inhibitor of FLICE; MACH-related inducer of toxicity; MRIT; testis secretory sperm-binding protein Li 233m; Usurpin; usurpin beta

Gene Aliases: c-FLIP; c-FLIPL; c-FLIPR; c-FLIPS; CASH; CASP8AP1; Casper; CFLAR; CLARP; FLAME; FLAME-1; FLAME1; FLIP; I-FLICE; MRIT

UniProt ID: (Human) O15519

Entrez Gene ID: (Human) 8837

Molecular Function: cysteine protease enzyme modulator hydrolase protease protease inhibitor