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CHD3 Recombinant Mouse Monoclonal Antibody, AbFlex®

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CHD3 Antibody in Western Blot (WB) — AbFlex® CHD3 antibody (rAb) tested by Western blot. 15 µg of Drosophila Schneider Cell nuclear extract was run on SDS-PAGE and probed with AbFlex CHD3 antibody at 0.5 µg/ml

CHD3 Antibody in Immunoprecipitation (IP) — AbFlex CHD3 antibody (rAb) tested antibody tested by immunoprecipitation. Drosophila Schneider cell nuclear extract was immunoprecipitated using 4 µg of AbFlex CHD3 antibody (Lane 1) or an unrelated AbFlex antibody (Lane 2). Protein from IP was probed by Western blot using AbFlex CHD3 antibody (2 µg/ml).

Product Details

91273

Applications	Tested Dilution	Publications
Western Blot (WB)	0.5-2 µg/mL	-
Immunoprecipitation (IP)	4 µg	-

Product Specifications
Species Reactivity	Fruit fly
Host/Isotype	Mouse / IgG2a
Class	Recombinant Monoclonal
Type	Antibody
Immunogen	This antibody was raised against a synthetic peptide corresponding to amino acid residues 865-885 of Drosophila.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	140mM HEPES/32mM sodium acetate, pH 7.5, with 30% glycerol, 70mM NaCl
Contains	0.035% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2793829

Target Information

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.