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CHD4 Antibody (MA5-44323) in WB
Western Blot of CHD4 in whole cell lysate (50 µg) from MCF-7, A-549, HeLa, K-562, Jurkat, and RKO cells. Samples were treated with NETN lysis buffer, incubated with CHD4 monoclonal antibody (Product # MA5-44324) at a dilution of 1:1000, followed by HRP-conjugated goat anti-rabbit IgG (exposure time of 10 seconds). Lower Panel: Rabbit anti-GAPDH.
Product Details
MA5-44323
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR066G
Immunogen
Between Residue 25 And 75
Conjugate
Form
Liquid
Concentration
40 µg/mL
Purification
purified
Storage buffer
BBS, pH 7.8-8.6, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
2-8°C
Shipping conditions
Wet ice
RRID
AB_2926453
Target Information
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase CHD4; CHD-4; Chromodomain-helicase-DNA-binding protein 4; Mi-2 autoantigen 218 kDa protein; Mi2-beta; MI2B
Gene Aliases: CHD-4; CHD4; Mi-2b; Mi2-BETA
UniProt ID: (Human) Q14839
Entrez Gene ID: (Human) 1108
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II repressing transcription factor binding
DNA binding
ATP-dependent DNA helicase activity
histone deacetylase activity
protein binding
ATP binding
microtubule binding
zinc ion binding
nucleosomal DNA binding
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
histone deacetylation
DNA duplex unwinding
ATP-dependent chromatin remodeling
spindle assembly
terminal button organization
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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