OriGene

CHGA Monoclonal Antibody (OTI2E12), TrueMAB™

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CHGA Antibody in Immunofluorescence (IF) — Anti-CHGA mouse monoclonal antibody (TA506098) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHGA(RC200492).

CHGA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-CHGA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506098)

CHGA Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHGA (RC200492, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CHGA. Positive lysates LY400511 (100 µg) and LC400511 (20 µg) can be purchased separately from OriGene.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:200-1:4000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI2E12

Immunogen

Full length human recombinant protein of human CHGA produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

Chromogranin A (CHGA) is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocine cells. CHGA is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides act as autocine or paracrine negative modulators of the neuroendocrine system. CHGA is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AL-11; AL26; Beta-granin; betagranin (N-terminal fragment of chromogranin A); Catestatin; CgA; chromofungin; Chromogranin-A; CTNNB; DKFZp686D02253; EA-92; ER-37; ES-43; FLJ25606; FLJ37923; GE-25; GR-44; GV-19; LF-19; MGC105435; p-Glu serpinin precursor; Pancreastatin; parathyroid secretory protein 1; Pituitary secretory protein I; Serpinin; Serpinin-RRG; SL21; SP-I; SS-18; Vasostatin I; Vasostatin II; Vasostatin-1; Vasostatin-2; WA-8; WE-14

Gene Aliases: CGA; CHGA; ChrA

UniProt ID: (Human) P10645, (Mouse) P26339

Entrez Gene ID: (Human) 1113, (Mouse) 12652

Molecular Function: peptide hormone signaling molecule