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CHM Antibody (MA5-44856) in ICC/IF
Immunocytochemistry analysis of CHM in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in CHM Monoclonal antibody (Product # MA5-44856) using a dilution of 1:50 in 2% negative goat serum overnight at 4 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI.
Product Details
MA5-44856
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE61-83
Immunogen
Synthetic peptide within human CHM aa 36-85/653.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2931312
Target Information
Substrate-binding subunit of the Rab geranylgeranyltransferase (GGTase) complex. Binds unprenylated Rab proteins and presents the substrate peptide to the catalytic component B composed of RABGGTA and RABGGTB, and remains bound to it after the geranylgeranyl transfer reaction. The component A is thought to be regenerated by transferring its prenylated Rab back to the donor membrane. Besides, a pre-formed complex consisting of CHM and the Rab GGTase dimer (RGGT or component B) can bind to and prenylate Rab proteins; this alternative pathway is proposed to be the predominant pathway for Rab protein geranylgeranylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: choroideraemia protein homolog; choroideremia (Rab escort protein 1); Choroideremia protein; Choroideremia protein homolog; choroidermia; Rab escort protein 1; Rab proteins geranylgeranyltransferase component A 1; REP-1; TCD protein
Gene Aliases: CHM; DXS540; GGTA; HSD-32; REP-1; REP1; TCD
UniProt ID: (Human) P24386, (Rat) P37727
Entrez Gene ID: (Human) 1121, (Mouse) 12662, (Rat) 24942
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