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Invitrogen
CHMP2B Recombinant Rabbit Monoclonal Antibody (JE54-35)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 12
CHMP2B Antibody (MA5-36184) in ICC/IF
Immunofluorescence of CHMP2B was performed using U2OS wild-type and CHMP2B KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the CHMP2B recombinant mo... View More
Product Details
MA5-36184
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE54-35
Immunogen
Recombinant protein within Human CHMP2B aa 103-213
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2890433
Target Information
CHMP2B, also known as CHMP2.5 or vacuolar protein-sorting-associated protein 2-2, is a 213 amino acid cytosolic protein. Widely expressed in brain, heart, skeletal muscle, small intestine, pancreas, lung, placenta and leukocytes, CHMP2B associates directly with CHMP2A and vps4 for the disassembly of the ESCRT-III complex. Defects in the gene encoding CHMP2B have been shown to cause chromosome 3-linked frontotemporal dementia (FTD3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Charged multivesicular body protein 2b; CHMP family; CHMP2.5; CHMP2b; chromatin modifying protein 2B; Chromatin-modifying protein 2b; hVps2-2; Vacuolar protein sorting-associated protein 2-2; vacuolar protein-sorting-associated protein 2-2; VPS2 homolog B; Vps2-2
Gene Aliases: 1190006E07Rik; ALS17; CGI-84; CHMP2.5; CHMP2B; DMT1; RGD1306781; VPS2-2; VPS2B
UniProt ID: (Human) Q9UQN3, (Mouse) Q8BJF9
Entrez Gene ID: (Human) 25978, (Mouse) 68942, (Rat) 363720
cell separation after cytokinesis
autophagy
nucleus organization
endosome organization
mitotic metaphase plate congression
regulation of centrosome duplication
protein transport
endosomal transport
viral life cycle
multivesicular body assembly
viral budding via host ESCRT complex
cognition
neuron cellular homeostasis
multivesicular body sorting pathway
cell-cell adhesion
regulation of mitotic spindle assembly
positive regulation of viral release from host cell
ESCRT III complex disassembly
transport
vacuolar transport
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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