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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||A bacterially expressed, mouse CHOP fusion protein.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1/100|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-250 detects CHOP protein in human and mouse samples.
MA1-250 has successfully been used in Western blot, immunoprecipitation, immunocytochemistry, and immunofluorescence procedures. By Western blot, this antibody detects an ~31 kDa protein representing CHOP from primary human fibroblast extract.
The MA1-250 immunizing protein corresponds to a bacterially expressed, mouse CHOP fusion protein. Clone 9C8 has been shown to recognize an epitope in the N-terminal region of CHOP.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
CHOP (also known as GADD153, Growth Arrest and DNA Damage Inducible Protein 153, and C/EBP Homology Protein) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets.
Under normal cellular conditions this protein is not expressed in detectable levels, but is highly upregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Identification of 5-nitrofuran-2-amide derivatives that induce apoptosis in triple negative breast cancer cells by activating C/EBP-homologous protein expression.
MA1-250 was used in western blot to identify compounds that induce CHOP expression and investigate how they induce apoptosis in cancer cells.
|Duan H,Li Y,Lim HY,Wang W||Bioorganic & medicinal chemistry (23:4514)||2015|
Preventing proteostasis diseases by selective inhibition of a phosphatase regulatory subunit.
MA1-250 was used in western blot to show that Sephin1, a selective inhibitor of protein phosphatase 1, protects mice from protein-misfolding disease.
|Das I,Krzyzosiak A,Schneider K,Wrabetz L,D'Antonio M,Barry N,Sigurdardottir A,Bertolotti A||Science (New York, N.Y.) (348:239)||2015|
Pharmaceutical integrated stress response enhancement protects oligodendrocytes and provides a potential multiple sclerosis therapeutic.
MA1-250 was used in western blot to determine the effects of guanabenz treatment on elapsing-remitting experimental autoimmune encephalomyelitis.
|Way SW,Podojil JR,Clayton BL,Zaremba A,Collins TL,Kunjamma RB,Robinson AP,Brugarolas P,Miller RH,Miller SD,Popko B||Nature communications (6:null)||2015|
Impact of aging on heat shock protein expression in the substantia nigra and striatum of the female rat.
MA1-250 was used in western blot to study age-dependent changes in the expression of a number of heat shock proteins in female rat substantia nigra and striatum
|Gleixner AM,Pulugulla SH,Pant DB,Posimo JM,Crum TS,Leak RK||Cell and tissue research (357:43)||2014|
TUSC3 loss alters the ER stress response and accelerates prostate cancer growth in vivo.
MA1-250 was used in western blot to study the role of the ER stress response in the mechanism by which loss of TUSC3 promotes the growth of prostate cancer
|Horak P,Tomasich E,Vaňhara P,Kratochvílová K,Anees M,Marhold M,Lemberger CE,Gerschpacher M,Horvat R,Sibilia M,Pils D,Krainer M||Scientific reports (4:null)||2014|
Xanthohumol impairs autophagosome maturation through direct inhibition of valosin-containing protein.
MA1-250 was used in western blot to study the role of valosin-containing protein in the mechanism by which xanthohumol inhibits the maturation of autophagosomes
|Sasazawa Y,Kanagaki S,Tashiro E,Nogawa T,Muroi M,Kondoh Y,Osada H,Imoto M||ACS chemical biology (7:892)||2012|
The transcription factor c-Jun protects against sustained hepatic endoplasmic reticulum stress thereby promoting hepatocyte survival.
MA1-250 was used in western blot to investigate the protective effect of c-Jun against hepatic endoplasmic reticulum stress
|Fuest M,Willim K,MacNelly S,Fellner N,Resch GP,Blum HE,Hasselblatt P||Hepatology (Baltimore, Md.) (55:408)||2012|
Protein aggregation of SERCA2 mutants associated with Darier disease elicits ER stress and apoptosis in keratinocytes.
MA1-250 was used in western blot to study the effect of aggregation of SERCA2 mutants associated with Darier disease on ER stress and keratinocyte apoptosis
|Wang Y,Bruce AT,Tu C,Ma K,Zeng L,Zheng P,Liu Y,Liu Y||Journal of cell science (124:3568)||2011|
Screening of cell death genes with a mammalian genome-wide RNAi library.
MA1-250 was used in western blot to identify genes related to cell death by using a mammalian genome-wide RNAi library
|Tsujii H,Eguchi Y,Chenchik A,Mizutani T,Yamada K,Tsujimoto Y||Journal of biochemistry (148:157)||2010|
DHA alters expression of target proteins of cancer therapy in chemotherapy resistant SW620 colon cancer cells.
MA1-250 was used in western blot to investigate the effect of DHA on specific colon cancer treatment by chemicals
|Slagsvold JE,Pettersen CH,Størvold GL,Follestad T,Krokan HE,Schønberg SA||Nutrition and cancer (62:611)||2010|
Cadmium induces Wnt signaling to upregulate proliferation and survival genes in sub-confluent kidney proximal tubule cells.
MA1-250 was used in western blot to investigate the effect of cadmium ion on Wnt signaling in kidney proximal tubule cells
|Chakraborty PK,Lee WK,Molitor M,Wolff NA,Thévenod F||Molecular cancer (9:null)||2010|
Regulated increase in folding capacity prevents unfolded protein stress in the ER.
MA1-250 was used in western blot to investigate the change of the folding capacity of ER due to thyroid stimulating hormone activation in thyrocytes
|Christis C,Fullaondo A,Schildknegt D,Mkrtchian S,Heck AJ,Braakman I||Journal of cell science (123:787)||2010|
Methods for monitoring endoplasmic reticulum stress and the unfolded protein response.
MA1-250 was used in western blot to evaluate different methods for the detection of endoplasmic reticulum stress and the unfolded protein response
|Samali A,Fitzgerald U,Deegan S,Gupta S||International journal of cell biology (2010:null)||2010|
Effects of ubiquilin 1 on the unfolded protein response.
MA1-250 was used in western blot to investigate the influence of ubiquilin 1 on the unfolded protein response and its role in Alzheimer disease
|Lu A,Hiltunen M,Romano DM,Soininen H,Hyman BT,Bertram L,Tanzi RE||Journal of molecular neuroscience : MN (38:19)||2009|
An upstream open reading frame regulates translation of GADD34 during cellular stresses that induce eIF2alpha phosphorylation.
MA1-250 was used in western blot to study the role of an upstream ORF in the transcritional regulation of GADD34 under conditions that induce phosphorylation of eIF2alpha
|Lee YY,Cevallos RC,Jan E||The Journal of biological chemistry (284:6661)||2009|
Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses.
MA1-250 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse
|Gramolini AO,Kislinger T,Alikhani-Koopaei R,Fong V,Thompson NJ,Isserlin R,Sharma P,Oudit GY,Trivieri MG,Fagan A,Kannan A,Higgins DG,Huedig H,Hess G,Arab S,Seidman JG,Seidman CE,Frey B,Perry M,Backx PH,Liu PP,MacLennan DH,Emili A||Molecular & cellular proteomics : MCP (7:519)||2008|
CHOP-Dependent stress-inducible expression of a novel form of carbonic anhydrase VI.
MA1-250 was used in western blot to investigate the role of carbonic anhydrase VI during CHOP-dependent expression.
|Sok J,Wang XZ,Batchvarova N,Kuroda M,Harding H,Ron D||Molecular and cellular biology (19:495)||1999|
Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153).
MA1-250 was used in western blot to investigate how the stress-induced protein CHOP affects adipogenesis
|Batchvarova N,Wang XZ,Ron D||The EMBO journal (14:4654)||1995|
CD36 deletion improves recovery from spinal cord injury.
MA1-250 was used in immunocytochemistry to study the mechanisms underlying the improved recovery from spinal cord injury of mice deleted for CD36
|Myers SA,Andres KR,Hagg T,Whittemore SR||Experimental neurology (256:25)||2014|
Oxidized lipids activate autophagy in a JNK-dependent manner by stimulating the endoplasmic reticulum stress response.
MA1-250 was used in immunocytochemistry to study the roles of JNK and the ER stress response in the activation of autophagy by oxidized lipids
|Haberzettl P,Hill BG||Redox biology (1:56)||2013|
Cloning of mammalian Ire1 reveals diversity in the ER stress responses.
MA1-250 was used in immunocytochemistry to demonstrate that Ire1 plays a role in multiple facets of the ER stress-response
|Wang XZ,Harding HP,Zhang Y,Jolicoeur EM,Kuroda M,Ron D||The EMBO journal (17:5708)||1998|
C/EBP Homology Protein; C/EBP homoologous protein 10; C/EBP zeta; c/EBP-homologous protein 10; CCAAT/enhancer-binding protein homologous protein; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; GADD153; Growth Arrest and DNA Damage Inducible Protein 153; growth arrest and DNA damage-inducible protein GADD153; growth arrest and DNA-damage-inducible protein GADD153
CEBPZ; CHOP; CHOP-10; CHOP10; DDIT3; GADD153