Invitrogen

CHRNA6 Monoclonal Antibody (5B6G8)

View all (9) CHRNA6 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CHRNA6 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CHRNA6 in Hela cells (green). Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:100-1:500 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

CHRNA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CHRNA6 in paraffin-embedded bladder cancer tissue. Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:200-1:1000 followed by DAB staining.

CHRNA6 Antibody in Western Blot (WB) — Western blot analysis of CHRNA6 in HEK293 (1) and CHRNA6 cell lysate. Samples were incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:500-1:2000.

CHRNA6 Antibody in Flow Cytometry (Flow) — Flow cytometry of CHRNA6 in SK-N-SH cells (green). Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:200-1:400 followed by negative control (red).

CHRNA6 Antibody in ELISA (ELISA) — ELISA analysis of CHRNA6 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:10,000.

Product Details

MA5-31689

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:500

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B6G8

Immunogen

Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. coli.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2787313

Product Specific Information

MA5-31689 has been tested in indirect ELISA.

Target Information

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acetylcholine receptor; acetylcholine receptor, nicotinic, alpha 6 (neuronal); cholinergic receptor; cholinergic receptor, nicotinic alpha 6; cholinergic receptor, nicotinic, alpha 6 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 6; Neuronal acetylcholine receptor subunit alpha-6

Gene Aliases: CHNRA6; CHRNA6

UniProt ID: (Human) Q15825

Entrez Gene ID: (Human) 8973

Molecular Function: ligand-gated ion channel

Function(s)

transport signal transduction chemical synaptic transmission synaptic transmission, cholinergic neuromuscular synaptic transmission regulation of dopamine secretion response to nicotine membrane depolarization cation transmembrane transport

Process(es)

acetylcholine-activated cation-selective channel activity ligand-gated ion channel activity acetylcholine receptor activity acetylcholine binding