Invitrogen

CHRNA6 Monoclonal Antibody (5B6G8)

Product Image Gallery

CHRNA6 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CHRNA6 in Hela cells (green). Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:100-1:500 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

CHRNA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CHRNA6 in paraffin-embedded bladder cancer tissue. Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:200-1:1000 followed by DAB staining.

CHRNA6 Antibody in Western Blot (WB) — Western blot analysis of CHRNA6 in HEK293 (1) and CHRNA6 cell lysate. Samples were incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:500-1:2000.

CHRNA6 Antibody in Flow Cytometry (Flow) — Flow cytometry of CHRNA6 in SK-N-SH cells (green). Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:200-1:400 followed by negative control (red).

CHRNA6 Antibody in ELISA (ELISA) — ELISA analysis of CHRNA6 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:10,000.

Product Details

MA5-31689

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:200-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Flow Cytometry (Flow)	1:200-1:400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	5B6G8
Immunogen	Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. coli. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2787313

Target Information

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: acetylcholine receptor; acetylcholine receptor, nicotinic, alpha 6 (neuronal); cholinergic receptor; cholinergic receptor, nicotinic alpha 6; cholinergic receptor, nicotinic, alpha 6 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 6; Neuronal acetylcholine receptor subunit alpha-6

Gene Aliases: CHNRA6; CHRNA6

UniProt ID: (Human) Q15825

Entrez Gene ID: (Human) 8973

Molecular Function: ligand-gated ion channel