Invitrogen

CHRNA6 Monoclonal Antibody (5B6G8)

Product Image Gallery

CHRNA6 Antibody in Western Blot (WB) — Western blot analysis of CHRNA6 in HEK293 (1) and CHRNA6 cell lysate. Samples were incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:500-1:2000.

CHRNA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CHRNA6 in paraffin-embedded bladder cancer tissue. Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:200-1:1000 followed by DAB staining.

CHRNA6 Antibody in Immunofluorescence (IF) — Immunocytochemistry analysis of CHRNA6 in Hela cells (green). Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:100-1:500 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

CHRNA6 Antibody in Flow Cytometry (Flow) — Flow cytometry of CHRNA6 in SK-N-SH cells (green). Sample was incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:200-1:400 followed by negative control (red).

CHRNA6 Antibody in ELISA (ELISA) — ELISA analysis of CHRNA6 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CHRNA6 monoclonal antibody (Product # MA5-31689) using a dilution of 1:10,000.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1000

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5B6G8

Immunogen

Purified recombinant fragment of human CHRNA6 (AA: 26-239) expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2787313

Target Information

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: acetylcholine receptor; acetylcholine receptor, nicotinic, alpha 6 (neuronal); cholinergic receptor; cholinergic receptor, nicotinic alpha 6; cholinergic receptor, nicotinic, alpha 6 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 6; Neuronal acetylcholine receptor subunit alpha-6

Gene Aliases: CHNRA6; CHRNA6

UniProt ID: (Human) Q15825

Entrez Gene ID: (Human) 8973

Molecular Function: GABA receptor acetylcholine receptor ion channel ligand-gated ion channel receptor transporter