Invitrogen

CHRNE Monoclonal Antibody (5F11G8)

Product Image Gallery

CHRNE Antibody in Western Blot (WB) — Western blot analysis of CHRNE in HEK293 (1) and CHRNE cell lysate. Samples were incubated with CHRNE monoclonal antibody (Product # MA5-31698) using a dilution of 1:500-1:2000.

CHRNE Antibody in Flow Cytometry (Flow) — Flow cytometry of CHRNE in SK-N-SH cells (green). Sample was incubated with CHRNE monoclonal antibody (Product # MA5-31698) using a dilution of 1:200-1:400 followed by negative control (red).

CHRNE Antibody in ELISA (ELISA) — ELISA analysis of CHRNE in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CHRNE monoclonal antibody (Product # MA5-31698) using a dilution of 1:10,000.

Product Details

Tested Applications

Dilution

ELISA (ELISA)

1:10,000

Flow Cytometry (Flow)

1:200-1:400

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

5F11G8

Immunogen

Purified recombinant fragment of human CHRNE (AA: extra 21-239) expressed in E. coli.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Target Information

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: acetylcholine receptor epsilon; Acetylcholine receptor subunit epsilon; acetylcholine receptor, nicotinic, epsilon (muscle); AchR epsilon subunit; ACHRE; cholinergic receptor, nicotinic epsilon; cholinergic receptor, nicotinic, epsilon (muscle); cholinergic receptor, nicotinic, epsilon polypeptide; CMS1D; CMS1E; CMS2A; CMS4A; CMS4B; CMS4C; epsilon-subunit; FCCMS; gene for acetylcholine receptor epsilon subunit; SCCMS

Gene Aliases: ACHRE; CHRNE; CMS1D; CMS1E; CMS2A; CMS4A; CMS4B; CMS4C; FCCMS; SCCMS

UniProt ID: (Human) Q04844, (Rat) P09660

Entrez Gene ID: (Human) 1145, (Rat) 29422

Molecular Function: GABA receptor acetylcholine receptor ion channel ligand-gated ion channel receptor transporter