Abnova

CHUK Monoclonal Antibody (1F8)

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CHUK Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. Antibody concentration is 10 µg/mL.

CHUK Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged CHUK is 0.03 ng/mL as a capture antibody.

CHUK Antibody in Proximity Ligation Assay (PLA) (PLA) — Proximity Ligation Analysis of protein-protein interactions between PRKCB and CHUK. HeLa cells were stained with anti-PRKCB rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Details

H00001147-M02

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	-
Immunocytochemistry (ICC/IF)	10 µg/mL	-
ELISA (ELISA)	0.03 ng/mL	-
in situ PLA (PLA)	1:1200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	1F8
Immunogen	CHUK (NP_001269, 646 a.a. approximately 745 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	See Label
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: RQKEIWHLLK IACTQSSARS LVGSSLEGAV TPQTSAWLPP TSAEHDHSLS CVVTPQDGET SAQMIEENLN CLGHLSTIIH EANEEQGNSM MNLDWSWLTE

Target Information

Inhibitor of Nuclear factor Kappa-B kinase subunit alpha (IKK alpha) is encoded by the CHUK gene and located on chromosome 10 in humans. It is also known as IKK1 or CHUK (Conserved helix-loop-helix ubiquitous kinase). IKK alpha belongs to the family of serine threonine protein kinase and forms a part of IKK complex. The IKK complex is required for the activation of NF-kB with IKK alpha specifically known to be involved in the non-canocial pathway for NF-kB activation. IKK alpha along with IKK beta and a regulatory protein NEMO (IKK gamma) phosphorylates IkB proteins which targets the later for ubiquitin mediated degradation. It is involved with several cellular processes with extensive studies on its role in inflammation and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Conserved helix-loop-helix ubiquitous kinase; EC 2.7.11.10; FLJ40509; I-kappa-B kinase 1; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; ikappaB kinase; IkB kinase alpha subunit; IKBKB; IKK-a kinase; IKK-B; IKK-beta; IKK1; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit alpha; kinase beta; MGC131801; NFKBIKA; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; Transcription factor 16

Gene Aliases: CHUK; IKBKA; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16

UniProt ID: (Human) O15111

Entrez Gene ID: (Human) 1147

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme