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CHX10 Antibody (MA5-38025) in WB
Western blot analysis of CHX10 in extracts of various cell lines. Samples were incubated with CHX10 Monoclonal antibody (Product # MA5-38025) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-38025
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2354
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 262-361 of human CHX10 (P58304)
Conjugate
Form
Liquid
Concentration
3 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897943
Product Specific Information
Positive Samples: Mouse spinal cord, Mouse brain, Rat spinal cord, Rat brain
Immunogen sequence: AAAESGRKPE GERQALPKLD KMEQDERGPD AQAAISQEEL RENSIAVLRA KAQEHSTKVL GTVSGPDSLA RSTEKPEEEE AMDEDRPAER LSPPQLEDMA
Target Information
CHX10 encodes a homeobox protein originally described as a retina-specific transcription factor. Mutations in this gene are associated with microphthalmia, cataracts and iris abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C. elegans ceh-10 homeo domain containing homolog; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10; ocular retardation; Visual system homeobox 2
Gene Aliases: Chx10; Hox-10; Hox10; or; Vsx2
UniProt ID: (Mouse) Q61412
Entrez Gene ID: (Mouse) 12677, (Rat) 171360
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
positive regulation of cell proliferation
negative regulation of cell proliferation
camera-type eye development
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
retinal bipolar neuron differentiation
retina morphogenesis in camera-type eye
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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