|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues L(10) L Y P K S L S R H V S V R T S(25) of human CIDE-3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500- 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-4316 detects the human CIDE-3 protein in human samples. Expected to recognize mouse samples due to 93% sequence homology.
PA1-4316 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~27 kDa protein representing human CIDE-3 in HepG2 cell lysate.
The PA1-4316 immunogen is a synthetic peptide corresponding to residues L(10) L Y P K S L S R H V S V R T S(25) of human CIDE-3. This antibody shows cross-reactivity to FSP27, which is the mouse homolog of CIDE-3, with a sequence identity of 93%.
Maintain at 4°C for 3 months or at -20°C for longer periods. Avoid repeated freeze-thaw cycles.
DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF). DFF is composed of two subunits, a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45). Recently a novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified. Human CIDE-3 is a novel member of CIDEs. There are 2 transcripts, CIDE-3 and CIDE-3alpha, were present in HepG2 and A375 cells. Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumours, CIDE-3 may play an important role in prevention of tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
FoxO1 antagonist suppresses autophagy and lipid droplet growth in adipocytes.
PA1-4316 was used in western blot to learn suppression of autophagy and lipid droplet growth in adipocytes by FoxO1 antagonist
|Liu L,Zheng LD,Zou P,Brooke J,Smith C,Long YC,Almeida FA,Liu D,Cheng Z||Cell cycle (Georgetown, Tex.) (15:2033)||2016|