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Invitrogen
CIP2A Monoclonal Antibody (2G10)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
CIP2A Antibody (MA1-46001) in ICC/IF
Immunofluorescence analysis of CIP2A was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CIP2A Monoclonal Antibody (2G10) (Product # MA1-46001) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti- Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A2817... View More
Product Details
MA1-46001
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
2G10
Immunogen
A C-terminal portion of the human p90 protein.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2130794
Product Specific Information
Suggested positive control: Hela whole cell extract, antigen standard for KIAA1524 (transient overexpression lysate).
Target Information
Inhibition of protein phosphatase 2A (PP2A) activity has been identified as a prerequisite for the transformation of human cells. The protein, designated Cancerous Inhibitor of PP2A (CIP2A), interacts directly with the oncogenic transcription factor c-Myc, inhibits PP2A activity toward c-Myc serine 62 (S62), and thereby prevents c-Myc proteolytic degradation. In addition to its function in c-Myc stabilization, CIP2A promotes anchorage-independent cell growth and in vivo tumor formation. The oncogenic activity of CIP2A is demonstrated by transformation of human cells by overexpression of CIP2A. Importantly, CIP2A is overexpressed in two common human malignancies, head and neck squamous cell carcinoma (HNSCC) and colon cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cancerous inhibitor of PP2A; cancerous inhibitor of protein phosphatase 2A; p90 autoantigen; p90 autoantigen homolog; Protein CIP2A; similar to RIKEN cDNA C330027C09
Gene Aliases: AA408511; AU018569; CIP2A; KIAA1524; p90
UniProt ID: (Mouse) Q8BWY9, (Human) Q8TCG1
Entrez Gene ID: (Mouse) 224171, (Human) 57650, (Rat) 360711
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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