Invitrogen

CLASP1 Recombinant Rabbit Monoclonal Antibody (JE43-19)

View all (11) CLASP1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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CLASP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CLASP1 in SH-SY-5Y cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with CLASP1 monoclonal antibody (Product # MA5-34834) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.

CLASP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CLASP1 in paraffin-embedded human small intestine tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with CLASP1 monoclonal antibody (Product # MA5-34834) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).

CLASP1 Antibody in Western Blot (WB) — Western blot analysis of CLASP1 in Positive: Lane 1: Daudi, Lane 2: HepG2. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with CLASP1 monoclonal antibody (Product # MA5-34834), at a dilution of 1:500 (5% BSA, 2 hours), followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.

CLASP1 Antibody in Flow Cytometry (Flow) — Flow cytometry of CLASP1 in SiHa cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with CLASP1 monoclonal antibody (Product # MA5-34834) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (30 min) with a dilution of 1:500.

Product Details

MA5-34834

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JE43-19
Immunogen	Synthetic peptide within Human CLASP1 aa 1-50 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848742

Product Specific Information

Positive Control: Daudi, HepG2, SH-SY-5Y, SiHa, rat kidney tissue, human appendix tissue, human small intestine tissue, mouse colon tissue.

Target Information

CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CLIP-associating protein 1; Cytoplasmic linker-associated protein 1; hOrbit1; multiple asters 1; Multiple asters homolog 1; Protein Orbit homolog 1

Gene Aliases: CLASP1; KIAA0622; MAST1

UniProt ID: (Human) Q7Z460

Entrez Gene ID: (Human) 23332

Molecular Function: non-motor microtubule binding protein

Function(s)

G2/M transition of mitotic cell cycle microtubule cytoskeleton organization microtubule bundle formation vesicle targeting microtubule nucleation negative regulation of microtubule depolymerization Golgi organization mitotic spindle organization sister chromatid cohesion establishment or maintenance of cell polarity exit from mitosis regulation of gastrulation positive regulation of epithelial cell migration regulation of epithelial to mesenchymal transition astral microtubule organization microtubule organizing center organization negative regulation of microtubule polymerization or depolymerization positive regulation of microtubule polymerization microtubule anchoring establishment of mitotic spindle localization positive regulation of exocytosis establishment of spindle orientation cell division negative regulation of stress fiber assembly regulation of focal adhesion assembly regulation of microtubule cytoskeleton organization positive regulation of extracellular matrix disassembly establishment of epithelial cell polarity mitotic spindle assembly negative regulation of wound healing, spreading of epidermal cells positive regulation of basement membrane assembly involved in embryonic body morphogenesis

Process(es)

dystroglycan binding protein binding microtubule binding kinetochore binding microtubule plus-end binding

CLASP1 Recombinant Rabbit Monoclonal Antibody (JE43-19)

Product Details

MA5-34834

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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