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CLEC11A Antibody (500-P162BT-500UG) in WB
Western Blot: To detect Human SCGF-alpha by Western Blot analysis CLEC11A Polyclonal Antibody, Biotin (Product # 500-P162BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human SCGF-alpha is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Product Details
500-P162BT-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 33.9 kDa Recombinant Human SCGF-alpha
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
AA Sequence of recombinant protein: GHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ AAAQARCTAR GGSLAQPADR QQMEALTRYL RAALAPYNWP VWLGVHDRRA EGLYLFENGQ RVSFFAWHRS PRPELGAQPS ASPHPLSPDQ PNGGTLENCV AQASDDGSWW DHDCQRRLYY VCEFPF.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SCGF-alpha. Anti-Human SCGF-alpha-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human SCGF-alpha by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCGF-alpha (500-P162) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human SCGF-alpha.
Western Blot: To detect Human SCGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human SCGF-alpha is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
500-P162BT-1 mg will be provided as 2 x 500 µg
Target Information
This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: b SCGF; beta SCGF; betaSCGF; bSCGF; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3; C-type lectin domain family 11 member A; C-type lectin domain family 11, member A; C-type lectin superfamily member 3; hCG_22930; Lymphocyte secreted C-type lectin; lymphocyte secreted long form of C-type lectin; Osteolectin; p47; SCGF b; SCGF beta; SCGF β; SCGFb; SCGFbeta; SCGFβ; Stem cell growth factor; stem cell growth factor; lymphocyte secreted C-type lectin; β SCGF; βSCGF
Gene Aliases: CLEC11A; CLECSF3; LSLCL; P47; SCGF
UniProt ID: (Human) Q9Y240
Entrez Gene ID: (Human) 6320
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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