Immunofluorescent analysis of CNG Channel using Anti-CNG Channel Monoclonal Antibody (CNC 9C1) (Product# MA3-901) shows staining in Hela Cells. CNG Channel staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CNG Channel (Product# MA3-901) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||MBP-fusion protein of the C-terminal region of the a2 subunit (amino acids K581-D670)|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA3-901 detects CNG channel from human, mouse and rat samples.
MA3-901 has been successfully used in Western blotting and immunohistochemistry procedures. By Western blot MA3-901 detects a band representing CNG channel. MA3-901 is specific to the a2 subunit of the CNG channel.
The MA3-901 immunogen is a MBP-fusion protein of the C-terminal region of the a2 subunit (amino acids K581-D670)
The cyclic-nucleotide gated (CNG) ion channel is a cytoplasmic multi-subunit protein complex, consisting of 4 alpha and 2 beta subunits. Binding of cGMP or cAMP induces a conformational change which opens the channel, and allows it to function as a link between Ca2+ signaling systems G-protein coupled reactions. The CNG channel is an element of olfactory and visual signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A cGMP-signaling pathway in a subset of olfactory sensory neurons.
MA3-901 was used in immunohistochemistry to investigate the important role of CNG channel in a cGMP-signaling pathway in sensory neurons of the rat olfactory epithelium
|Meyer MR,Angele A,Kremmer E,Kaupp UB,Muller F||Proceedings of the National Academy of Sciences of the United States of America (97:10595)||2000|