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Western blot analysis of Mouse Spinal Cord Tissue Lysate using MA5-14692, CNPase primary antibody at a dilution of 1 ug/ml (lane 1). MA5-14692 staining of Rat Spinal Cord Tissue Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
|Tested species reactivity||Dog, Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human CNPase.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg for 10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||1:300|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Western Blot, this antibody has non-specific bands at 42 kDa and 67 kDa.
This antibody is predicted to react with orangutan based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Selective localization of bone marrow-derived ramified cells in the brain adjacent to the attachments of choroid plexus.
MA5-14692 was used in immunohistochemistry to study the brain localization of bone marrow-derived ramified cells
|Hasegawa-Ishii S,Shimada A,Inaba M,Li M,Shi M,Kawamura N,Takei S,Chiba Y,Hosokawa M,Ikehara S||Brain, behavior, and immunity (29:82)||2013|
Downregulation of CNPase in a MeCP2 deficient mouse model of Rett syndrome.
MA5-14692 was used in western blot to study the potential pathogenic role of reduced CNPase levels in a murine model of Rett syndrome that lacks MeCP2
|Wu W,Gu W,Xu X,Shang S,Zhao Z||Neurological research (34:107)||2012|
2', 3' cyclic nucleotide 3' phosphohydrolase; 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNP1, 2', 3' cyclic nucleotide 3' phosphohydrolase; CNPase; cyclic nucleotide phosphodiesterase 1
CNP; Cnp-1; CNP1; CNPase; CNPF; CNPI; CNPII