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CNTF Antibody (ANT-031-200UL) in ICC/IF
Expression of CNTF in rat C6 glioma cells - Immunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained with Anti-CNTF Antibody (#ANT-031), (1:200), followed by goat Anti-rabbit-DyLight-594 secondary Antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.
Product Details
ANT-031-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF, C-terminus
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ciliary neurotrophic factor; ciliary neurotropic factor; CNTF; H-CNTF
Gene Aliases: AI429687; CNTF; HCNTF
UniProt ID: (Human) P26441, (Mouse) P51642, (Rat) P20294
Entrez Gene ID: (Human) 1270, (Mouse) 12803, (Rat) 25707
signal transduction
positive regulation of cell proliferation
positive regulation of gene expression
growth
positive regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of neuron apoptotic process
negative regulation of photoreceptor cell differentiation
regulation of retinal cell programmed cell death
astrocyte activation
muscle organ morphogenesis
neuron development
positive regulation of axon regeneration
ciliary neurotrophic factor-mediated signaling pathway
JAK-STAT cascade
multicellular organism development
nervous system development
positive regulation of phosphatidylinositol 3-kinase signaling
cell differentiation
positive regulation of cell growth
positive regulation of MAPK cascade
positive regulation of JAK-STAT cascade
positive regulation of hormone secretion
protein heterooligomerization
regulation of resting membrane potential
membrane hyperpolarization
negative regulation of neuron death
response to light intensity
response to electrical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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