|Tested species reactivity||Human, Rat, Rabbit|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant, full length human CNTF protein expressed in E. coli.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1 µg/ml|
|Inhibition Assays (IA)||120 ug/ml|
|Neutralization (Neu)||120 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24825 detects CNTF from human samples. PA1-24825 is expected to cross react with rat (85% conserved) and rabbit (85% conserved) due to sequence homology.
PA1-24825 has been successfully used in neutralization, inhibition assays, and Western blot procedures. Neutralization: The ND50 is 120 ug/ml.
The PA1-24825 immunogen is recombinant, full length human CNTF protein expressed in E. coli.
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.