Bethyl Laboratories

COBL Polyclonal Antibody

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COBL Antibody in Immunoprecipitation (IP) — **Detection of human COBL by western blot of immunoprecipitates.** *Samples:* Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. *Antibodies:* Affinity purified rabbit anti-COBL antibody A305-679A-M (lot A305-679A-M-1) used for IP at 8 µl per reaction. COBL was also immunoprecipitated by rabbit anti-COBL antibody BL22740. For blotting immunoprecipitated COBL, A305-679A-M was used at 1:1000. *Detection:* Chemiluminescence with an exposure time of 3 minutes.

Product Details

A305-679A-M

Applications	Tested Dilution	Publications
Immunoprecipitation (IP)	8-15 µL/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 850 and 900 View antigen
Conjugate	Unconjugated
Form	liquid
Concentration	0.40 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS with 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Target Information

This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.