Invitrogen

COL11A2 Monoclonal Antibody (GT473)

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COL11A2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of COL11A2 in A431 cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with COL11A2 monoclonal antibody (Product # MA5-27738) using a dilution of 1:500, followed by Hoechst.

COL11A2 Antibody in Western Blot (WB) — Western blot analysis of COL11A2 was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Product Details

MA5-27738

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	GT473
Immunogen	Recombinant protein encompassing a sequence within the N-terminus region of human COL11A2. The exact sequence is proprietary. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735152

Product Specific Information

Positive Control: human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1)

Predicted Reactivity: Mouse (85%), Rat (85%), Bovine (93%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Collagen alpha-2(XI) chain; collagen, type XI, alpha 2; DAQB-79P13.8; HGNC:2187; pro-a2 chain of collagen type XI; procollagen, type XI, alpha 2; type XI collagen alpha2 chain

Gene Aliases: COL11A2; DFNA13; DFNB53; FBCG2; HKE5; PARP; STL3

UniProt ID: (Human) P13942, (Mouse) Q64739

Entrez Gene ID: (Human) 1302, (Mouse) 12815, (Rat) 294279

Molecular Function: extracellular matrix structural protein