Western blot analysis of TPL2 in whole cell extracts from HepG2 using a TPL2 recombinant rabbit monoclonal antibody (Product # 701352) at a dilution of 1ug/ml. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~53kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 282–293 of human COT/TPL2/MAP3K8|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
COT (Cancer Osaka thyroid oncogene) also known as MAP3K8/TPL2 (Mitogen Activated Protein kinase 8/Tumor Progression Locus 2). This oncogene is a member of the serine threonine protein kinase family and is localized in the cytoplasm. It is known to activate JNK kinase and MAP kinase pathways along with activating IK-B kinases aiding in nuclear production of NF-KappaB. The production of IL-2 and TNFalpha during T cell activation is also mediated by COT/MAP3K8/TPL2. In addition to this, COT/MAP3K8/TPL2 also aids in T-cell costimulation, signal transduction, and amino acid phosphorylation. In humans, the MAP3K8 gene encoding COT/MAP3K8/TPL2 is present on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.