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Immunofluorescence analysis of COX-1 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COX-1 (COX 111) Mouse Monoclonal Antibody (358100) at 2ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Sheep, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG2b, kappa|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A cyclooxygenase-2-dependent prostaglandin E2 biosynthetic system in the Golgi apparatus.
35-8100 was used in immunocytochemistry and western blot to investigate a prostaglandin E2 biosynthetic system in the Golgi apparatus
|Yuan C,Smith WL||The Journal of biological chemistry (290:5606)||2015|
|Human||Not Cited||Effect of ginger root on cyclooxygenase-1 and 15-hydroxyprostaglandin dehydrogenase expression in colonic mucosa of humans at normal and increased risk for colorectal cancer.||Jiang Y,Turgeon DK,Wright BD,Sidahmed E,Ruffin MT,Brenner DE,Sen A,Zick SM||European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP) (22:455)||2013|
The Ras signaling inhibitor LOX-PP interacts with Hsp70 and c-Raf to reduce Erk activation and transformed phenotype of breast cancer cells.
35-8100 was used in western blot to identify the interaction of LOX-PP with Hsp70 and c-Raf and investigate how this interaction affects Ras-induced MEK signaling.
|Sato S,Trackman PC,Mäki JM,Myllyharju J,Kirsch KH,Sonenshein GE||Molecular and cellular biology (31:2683)||2011|
|Rat||Not Cited||Amino acid-based ionic liquids: using XPS to probe the electronic environment via binding energies.||Hurisso BB,Lovelock KR,Licence P||Physical chemistry chemical physics : PCCP (13:17737)||2011|
COX-1; COX3; cyclooxygenase 3; cyclooxygenase-1; PCOX1; PGG/HS; PGH synthase 1; PGHS-1; PGHS1; PHS 1; PHS1; prostaglandin endoperoxide synthase; prostaglandin G/H synthase 1; prostaglandin G/H synthase and cyclooxygenase; prostaglandin H2 synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase); PTGHS
COX-1; Cox-3; COX1; COX3; PCOX1; PES-1; PGG/HS; PGHS-1; PGHS1; PHS 1; PHS1; PTGHS; PTGS1