|Tested species reactivity||Bovine, Human, Non-human primate, Pig, Rat, Zebrafish|
|Published species reactivity||Rat, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding Lys29 of human COX IV|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.
MA5-15078 was used in western blot to study the role of nuclear CLK-1 in regulating mitochondrial stress responses and aging.
|Monaghan RM,Barnes RG,Fisher K,Andreou T,Rooney N,Poulin GB,Whitmarsh AJ||Nature cell biology (17:782)||2015|
3,5-Diiodo-L-thyronine activates brown adipose tissue thermogenesis in hypothyroid rats.
MA5-15078 was used in immunohistochemistry - paraffin section to test if 3,5-Diiodo-l-thyronine could activate brown adipose tissue thermogenesis
|Lombardi A,Senese R,De Matteis R,Busiello RA,Cioffi F,Goglia F,Lanni A||PloS one (10:null)||2015|