|Tested species reactivity||Human, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human Cycloxygenase 2|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not cross-react with human COX1.
Cyclooxygenase (COX), also known as prostaglandin H2 synthase and Prostaglandin endoperoxide synthase, is a key enzyme in the conversion of arachidonic acid to Prostaglandin H2. Prostaglandin H2 is converted by other enzymes into inflammatory mediators prostaglandin (PG) D2, PGE2, PGF2a, PGI2 and Thromboxane A2. Thus, COX is a key enzyme in the production of inflammatory agents and is the target of intense research and drug discovery activities. There are two enzymes of COX, COX-1, and COX-2. COX-1 is constitutively produced in many (most) cell types. It is important in the gut for the production of prostaglandins, which inhibit gastric secretion. It can be induced in monocytes, macrophages and other cells. It is induced as part of the inflammatory response by Il-1beta and other cytokines. It is induced by growth factors such as EGF and PDGF. Expression is inhibited by glucorticosteroids such as cortisol and dexamethasone. Lipopolysaccharide in bacterial infections induces the COX-2. COX-2 is also found in elevated levels in synoviocytes from rheumatoid arthritis patients. The discomforts of imflammation such as pain and swelling are largely due to the action of prostaglandins produced by COX-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.