Immunofluorescence analysis of COX2 was performed using 70% confluent log phase HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COX2 Rabbit Polyclonal Antibody (PA516817) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to c-terminus of rat COX2|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3 - 5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-200|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16817 targets COX2 in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16817 immunogen is synthetic peptide corresponding to c-terminus of rat COX2.
COX2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model.
PA5-16817 was used in immunohistochemistry - paraffin section to assess the use of mitotic error-induced CIN and Shugoshin1 haplo insufficient mice as models to study chromosome instability in colon cancer
|Rao CV,Sanghera S,Zhang Y,Biddick L,Reddy A,Lightfoot S,Dai W,Yamada HY||Molecular carcinogenesis (55:600)||2016|
|Not Applicable||Not Cited||
Genistein improves 3-NPA-induced memory impairment in ovariectomized rats: impact of its antioxidant, anti-inflammatory and acetylcholinesterase modulatory properties.
PA5-16817 was used in immunohistochemistry - paraffin section to characterize how 3-NPA-induced memory impairment in ovariectomized rats is improved by Genistein and its anti-inflammatory, antioxidant, and acetylcholinesterase modulatory properties
|Menze ET,Esmat A,Tadros MG,Abdel-Naim AB,Khalifa AE||PloS one (10:null)||2015|
|Not Applicable||Not Cited||
PharmDB-K: Integrated Bio-Pharmacological Network Database for Traditional Korean Medicine.
PA5-16817 was used in western blot to present PharmDB-K, a database offering comprehensive information relating to TKM-associated drugs, disease indication, and protein relationships
|Lee JH,Park KM,Han DJ,Bang NY,Kim DH,Na H,Lim S,Kim TB,Kim DG,Kim HJ,Chung Y,Sung SH,Surh YJ,Kim S,Han BW||PloS one (10:null)||2015|
Aspirin blocks growth of breast tumor cells and tumor-initiating cells and induces reprogramming factors of mesenchymal to epithelial transition.
PA5-16817 was used in western blot to evaluate the effect of aspirin in mesenchymal to epithelial transition
|Maity G,De A,Das A,Banerjee S,Sarkar S,Banerjee SK||Laboratory investigation; a journal of technical methods and pathology (95:702)||2015|
Resveratrol suppresses 4-hydroxyestradiol-induced transformation of human breast epithelial cells by blocking I¿B kinaseß-NF-¿B signalling.
PA5-16817 was used in western blot to study the role of IKK-beta and NFkB in the mechanism by which resveratrol blocks human breast epithelial transformation induced by 4-hydroxyestradiol
|Park SA,Na HK,Surh YJ||Free radical research (46:1051)||2012|
Cyclooxygenase-2 expression and its prognostic significance in clear cell renal cell carcinoma.
PA5-16817 was used in immunohistochemistry and western blot to study the prognostic value of the immunohistochemical expression of COX-2 in patients with clear cell renal cell carcinoma
|Lee JW,Park JH,Suh JH,Nam KH,Choe JY,Jung HY,Chae JY,Moon KC||Korean journal of pathology (46:237)||2012|
The acid sphingomyelinase inhibitors block interferon-¿-induced serotonin uptake via a COX-2/Akt/ERK/STAT-dependent pathway in T cells.
PA5-16817 was used in western blot to study the role of sphingomyelinase inhibitors in serotonin uptake
|Su HC,Ma CT,Lin CF,Wu HT,Chuang YH,Chen LJ,Tsao CW||International immunopharmacology (11:1823)||2011|
Cyclooxygenase (COX)-1 activity precedes the COX-2 induction in Aß-induced neuroinflammation.
PA5-16817 was used in western blot to investigate the activation of cyclooxygenases in the neuroinflammation mediated by Abeta
|Dargahi L,Nasiraei-Moghadam S,Abdi A,Khalaj L,Moradi F,Ahmadiani A||Journal of molecular neuroscience : MN (45:10)||2011|
Immunohistochemical evaluation of COX-1 and COX-2 expression in keloid and hypertrophic scar.
PA5-16817 was used in immunohistochemistry to evaluate COX-1 and COX-2 expression in keloid and hypertrophic scars
|Abdou AG,Maraee AH,Saif HF||The American Journal of dermatopathology (36:311)||2014|
Comparative immunohistochemical assessment of cutaneous cyclooxygenase-2 enzyme expression in chronological aging and photoaging.
PA5-16817 was used in immunohistochemistry to perform a comparative study of the immunohistochemical expression of COX-2 in skin samples from individuals with chronologically or photo aged skin
|Habib MA,Salem SA,Hakim SA,Shalan YA||Photodermatology, photoimmunology and photomedicine (30:43)||2014|
Reappraisal of the therapeutic role of celecoxib in cholangiocarcinoma.
PA5-16817 was used in immunohistochemistry to evaluate the efficacy and safety of celecoxib to treat cholangiocarcinoma
|Yeh CN,Chiang KC,Juang HH,S Pang JH,Yu CS,Lin KJ,Yeh TS,Jan YY||PloS one (8:null)||2013|
|Not Applicable||Not Cited||
Multimechanistic antifibrotic effect of biochanin a in rats: implications of proinflammatory and profibrogenic mediators.
PA5-16817 was used in immunohistochemistry to assess the antifibrotic of biochanin A
|Breikaa RM,Algandaby MM,El-Demerdash E,Abdel-Naim AB||PloS one (8:null)||2013|
The effects of ulipristal on Bax/Bcl-2, cytochrome c, Ki-67 and cyclooxygenase-2 expression in a rat model with surgically induced endometriosis.
PA5-16817 was used in immunohistochemistry to use a rat model of endometriosis to study the effects of ulipristal on endometrial histology and the expression of Bax/Bcl-2, cytochrome c, Ki-67 and COX-2
|Huniadi CA,Pop OL,Antal TA,Stamatian F||European journal of obstetrics, gynecology, and reproductive biology (169:360)||2013|
Suppression of ß-catenin and cyclooxygenase-2 expression and cell proliferation in azoxymethane-induced colonic cancer in rats by rice bran phytic acid (PA).
PA5-16817 was used in immunohistochemistry to study the ability of phytic acid from rice bran to reduce the expression of beta-catenin and COX-2 and to inhibit colon cancer proliferation in a rat carcinogen-induced model
|Saad N,Esa NM,Ithnin H||Asian Pacific journal of cancer prevention : APJCP (14:3093)||2013|
A promise in the treatment of endometriosis: an observational cohort study on ovarian endometrioma reduction by N-acetylcysteine.
PA5-16817 was used in immunohistochemistry to study the efficacy of N-acetylcysteine therapy in women with ovarian endometriosis
|Porpora MG,Brunelli R,Costa G,Imperiale L,Krasnowska EK,Lundeberg T,Nofroni I,Piccioni MG,Pittaluga E,Ticino A,Parasassi T||Evidence-based complementary and alternative medicine : eCAM (2013:null)||2013|
Simvastatin treatment ameliorates injury of rat testes induced by cadmium toxicity.
PA5-16817 was used in immunohistochemistry to study the mechanisms underlying the protection against cadmium-induced testicular toxicity conferred by treatment with simvastatin
|Fouad AA,Albuali WH,Jresat I||Biological trace element research (153:269)||2013|
Therapeutic effect of coenzyme Q10 against experimentally-induced hepatocellular carcinoma in rats.
PA5-16817 was used in immunohistochemistry to study the therapeutic effects of coenzyme Q10 in a rat model of hepatocellular carcinoma
|Fouad AA,Al-Mulhim AS,Jresat I||Environmental toxicology and pharmacology (35:100)||2013|
Protective effects of nebivolol against cold restraint stress-induced gastric ulcer in rats: role of NO, HO-1, and COX-1,2.
PA5-16817 was used in immunohistochemistry to study the protective mechanism of nebivolol in a rat gastric ulcer model
|Morsy MA,Heeba GH,Abdelwahab SA,Rofaeil RR||Nitric oxide : biology and chemistry (27:117)||2012|
Inhibition of COX-2 in colon cancer modulates tumor growth and MDR-1 expression to enhance tumor regression in therapy-refractory cancers in vivo.
PA5-16817 was used in immunohistochemistry and western blot to study the beneficial effects on tumor growth and MDR-1 expression of pharmacological COX-2 inhibition in chemoresistant colorectal cancer
|Rahman M,Selvarajan K,Hasan MR,Chan AP,Jin C,Kim J,Chan SK,Le ND,Kim YB,Tai IT||Neoplasia (New York, N.Y.) (14:624)||2012|
Role of immunohistochemical cyclo-oxygenase-2 (COX-2) and osteocalcin in differentiating between osteoblastomas and osteosarcomas.
PA5-16817 was used in immunohistochemistry to study the differentiation of osteoblastomas and osteosarcomas based on the immunohistochemical measurement of COX-2 and osteocalcin
|El-Badawi ZH,Muhammad EM,Noaman HH||The Malaysian journal of pathology (34:15)||2012|
Cox-2 deletion in myeloid and endothelial cells, but not in epithelial cells, exacerbates murine colitis.
PA5-16817 was used in immunohistochemistry to investigate the involvement of COX2 expression in different cell types in the pathogenesis of colitis
|Ishikawa TO,Oshima M,Herschman HR||Carcinogenesis (32:417)||2011|
Tumor formation in a mouse model of colitis-associated colon cancer does not require COX-1 or COX-2 expression.
PA5-16817 was used in immunohistochemistry to investigate the influence of COX-1 or COX-2 on specific tumor formation in mice
|Ishikawa TO,Herschman HR||Carcinogenesis (31:729)||2010|