|Flow Cytometry (Flow)||1 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
|Western Blot (WB)||See 28 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 3 publications below|
|Immunocytochemistry (ICC)||See 7 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Miscellaneous PubMed (MISC)||See 12 publications below|
|Tested Species reactivity||Bovine, C. elegans, Goat, Hamster, Human, Mollusc, Mouse, Sheep, Rhesus monkey, Rat, Zebrafish|
|Published species reactivity||Rat , Mouse , Human , Guinea pig|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Human Complex IV subunit I|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage conditions||4° C, do not freeze|
For positive control, use Human heart mitochondria. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HEK293 cells.
Samples that are to be used in Western blotting with this antibody should NOT be boiled before loading onto the gel. Boiling of the sample will cause a loss of signal in Western blotting.
In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa).
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: COX IV-1; COX4; COX4-1; COXIV; Cytochrome c oxidase polypeptide IV; cytochrome c oxidase subunit 4 isoform 1 mitochondrial; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; Cytochrome c oxidase subunit IV isoform 1; cytochrome c oxidase, subunit 4a; cytochrome c oxidase, subunit IV; cytochrome c oxidase, subunit IVa; cytochrome c oxydase subunit 4
Gene Aliases: AL024441; bb02d03; BOS_17018; COX; COX IV-1; COX4; COX4-1; Cox4a; COX4I1; COXIV; COXIV-1; DKEY-265O13.1; IV-1; mg:bb02d03; zgc:110058
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