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|Tested species reactivity||Bovine, C. elegans, Goat, Hamster, Human, Mollusc, Mouse, Sheep, Rhesus monkey, Rat, Zebrafish|
|Published species reactivity||Rat, Mouse, Human, Guinea pig|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Human Complex IV subunit I|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For positive control, use Human heart mitochondria. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal colon. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HEK293 cells.
Samples that are to be used in Western blotting with this antibody should NOT be boiled before loading onto the gel. Boiling of the sample will cause a loss of signal in Western blotting.
In Western blot applications, this antibody detects a band at approximately 40 kDa (predicted MW: 57 kDa).
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Imbalanced OPA1 processing and mitochondrial fragmentation cause heart failure in mice.
459600 was used in western blot to study how OMA1 links mitochondrial morphology and cardiac metabolism.
|Wai T,García-Prieto J,Baker MJ,Merkwirth C,Benit P,Rustin P,Rupérez FJ,Barbas C,Ibañez B,Langer T||Science (New York, N.Y.) (350:null)||2015|
AMPKα is essential for acute exercise-induced gene responses but not for exercise training-induced adaptations in mouse skeletal muscle.
459600 was used in western blot to elucidate the role of AMPKα and -α2 subunits in acute exercise-induced mRNA responses.
|Fentz J,Kjøbsted R,Kristensen CM,Hingst JR,Birk JB,Gudiksen A,Foretz M,Schjerling P,Viollet B,Pilegaard H,Wojtaszewski JF||American journal of physiology. Endocrinology and metabolism (309:E900)||2015|
A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.
459600 was used in western blot to study the role of nuclear CLK-1 in regulating mitochondrial stress responses and aging.
|Monaghan RM,Barnes RG,Fisher K,Andreou T,Rooney N,Poulin GB,Whitmarsh AJ||Nature cell biology (17:782)||2015|
AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD.
459600 was used in western blot to test the requirement of AMP-activated protein kinase for exercise training-induced increases in skeletal muscle abundance of mitochondrial proteins.
|Brandauer J,Andersen MA,Kellezi H,Risis S,Frøsig C,Vienberg SG,Treebak JT||Frontiers in physiology (6:null)||2015|
Overexpression of TFAM or twinkle increases mtDNA copy number and facilitates cardioprotection associated with limited mitochondrial oxidative stress.
459600 was used in western blot to assess the role of mtDNA copy number in heart failure
|Ikeda M,Ide T,Fujino T,Arai S,Saku K,Kakino T,Tyynismaa H,Yamasaki T,Yamada K,Kang D,Suomalainen A,Sunagawa K||PloS one (10:null)||2015|
An MRPS12 mutation modifies aminoglycoside sensitivity caused by 12S rRNA mutations.
459600 was used in western blot to use an evolutionary approach to identify putative modifying factors involved in aminoglycoside-induced and non-syndromic hearing loss.
|Emperador S,Pacheu-Grau D,Bayona-Bafaluy MP,Garrido-Pérez N,Martín-Navarro A,López-Pérez MJ,Montoya J,Ruiz-Pesini E||Frontiers in genetics (5:null)||2015|
Endonuclease G preferentially cleaves 5-hydroxymethylcytosine-modified DNA creating a substrate for recombination.
459600 was used in western blot to study endonuclease G-mediated cleavage of 5hmC-modified DNA
|Robertson AB,Robertson J,Fusser M,Klungland A||Nucleic acids research (42:13280)||2014|
Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats.
459600 was used in western blot to determine the mechanisms underlying the decrease in hepatic cytochrome P-450 during ascorbic acid deficiency using scurvy-prone ODS rats.
|Kobayashi M,Hoshinaga Y,Miura N,Tokuda Y,Shigeoka S,Murai A,Horio F||Bioscience, biotechnology, and biochemistry (78:1060)||2014|
Independent and additive effects of atenolol and methionine restriction on lowering rat heart mitochondria oxidative stress.
459600 was used in western blot to study the effects on rat cardiac mitochondrial oxidative stress of simultaneous treatment with atenolol plus methionine restriction.
|Sanchez-Roman I,Gomez A,Naudí A,Jove M,Gómez J,Lopez-Torres M,Pamplona R,Barja G||Journal of bioenergetics and biomembranes (46:159)||2014|
Exercise training, but not resveratrol, improves metabolic and inflammatory status in skeletal muscle of aged men.
459600 was used in western blot to compare the effect of exercise training and resveratrol treatment on metabolic and inflammatory status in skeletal muscle of aged men
|Olesen J,Gliemann L,Biensø R,Schmidt J,Hellsten Y,Pilegaard H||The Journal of physiology (592:1873)||2014|
Effects of Roux-en-Y gastric bypass and ileal transposition surgeries on glucose and lipid metabolism in skeletal muscle and liver.
459600 was used in western blot to compare the effects of Roux-en Y gastric bypass and ileal transposition surgeries on glucose and lipid metabolism in muscle and liver.
|Pezeshki A,Chelikani PK||Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery (10:217)||2014|
Identification of mitochondrial dysfunction in Hutchinson-Gilford progeria syndrome through use of stable isotope labeling with amino acids in cell culture.
459600 was used in western blot to elucidate the mechanisms underlying cellular damage and senescence and accelerated aging in Hutchinson-Gilford progeria syndrome.
|Rivera-Torres J,Acín-Perez R,Cabezas-Sánchez P,Osorio FG,Gonzalez-Gómez C,Megias D,Cámara C,López-Otín C,Enríquez JA,Luque-García JL,Andrés V||Journal of proteomics (91:466)||2013|
GRSF1 regulates RNA processing in mitochondrial RNA granules.
459600 was used in western blot to study the localization of a GRSF isoform to the mitochondrial matrix and its role in RNA processing.
|Jourdain AA,Koppen M,Wydro M,Rodley CD,Lightowlers RN,Chrzanowska-Lightowlers ZM,Martinou JC||Cell metabolism (17:399)||2013|
Two weeks of metformin treatment enhances mitochondrial respiration in skeletal muscle of AMPK kinase dead but not wild type mice.
459600 was used in western blot to investigate the effect of metformin treatment on mitochondrial respiration in mouse skeletal muscle
|Kristensen JM,Larsen S,Helge JW,Dela F,Wojtaszewski JF||PloS one (8:null)||2013|
Local arginase inhibition during early reperfusion mediates cardioprotection via increased nitric oxide production.
459600 was used in western blot to study arginase in ischemic myocardium.
|Gonon AT,Jung C,Katz A,Westerblad H,Shemyakin A,Sjöquist PO,Lundberg JO,Pernow J||PloS one (7:null)||2012|
Role of PGC-1α in exercise and fasting-induced adaptations in mouse liver.
459600 was used in western blot to examine the role of PGC-α in fasting.
|Haase TN,Ringholm S,Leick L,Biensø RS,Kiilerich K,Johansen S,Nielsen MM,Wojtaszewski JF,Hidalgo J,Pedersen PA,Pilegaard H||American journal of physiology. Regulatory, integrative and comparative physiology (301:R1501)||2011|
The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction.
459600 was used in western blot to identify molecules involved in sperm-zona pellucida interactions.
|Dun MD,Smith ND,Baker MA,Lin M,Aitken RJ,Nixon B||The Journal of biological chemistry (286:36875)||2011|
Mutations in MTFMT underlie a human disorder of formylation causing impaired mitochondrial translation.
459600 was used in western blot to test the requirement of formylation for initiation in human mitochondria.
|Tucker EJ,Hershman SG,Köhrer C,Belcher-Timme CA,Patel J,Goldberger OA,Christodoulou J,Silberstein JM,McKenzie M,Ryan MT,Compton AG,Jaffe JD,Carr SA,Calvo SE,RajBhandary UL,Thorburn DR,Mootha VK||Cell metabolism (14:428)||2011|
Mutation in the mitochondrial tRNA(Val) causes mitochondrial encephalopathy, lactic acidosis and stroke-like episodes.
459600 was used in western blot to study the effects of the m.1630A G mutation in the mitochondrial tRNA gene.
|Glatz C,D'Aco K,Smith S,Sondheimer N||Mitochondrion (11:615)||2011|
Exome Sequencing of SLC30A2 Identifies Novel Loss- and Gain-of-Function Variants Associated with Breast Cell Dysfunction.
459600 was used in immunocytochemistry to assess the genetic variation of SLC30A2 associated with breast cell dysfunction
|Alam S,Hennigar SR,Gallagher C,Soybel DI,Kelleher SL||Journal of mammary gland biology and neoplasia (20:159)||2015|
Upregulation of mitochondrial Nox4 mediates TGF-β-induced apoptosis in cultured mouse podocytes.
459600 was used in immunocytochemistry to study the role of mitochochondrial Nox4 upregulation in the induction of cultured murine podicyte apoptosis by TGF-beta.
|Das R,Xu S,Quan X,Nguyen TT,Kong ID,Chung CH,Lee EY,Cha SK,Park KS||American journal of physiology. Renal physiology (306:F155)||2014|
Confocal laser endomicroscopy: a new gold standard for the assessment of mucosal healing in ulcerative colitis.
459600 was used in immunohistochemistry - paraffin section to test if endoscopically normal appearing colonic mucosa in patients with UC in remission have fully recovered using confocal laser endomicroscopy
|Macé V,Ahluwalia A,Coron E,Le Rhun M,Boureille A,Bossard C,Mosnier JF,Matysiak-Budnik T,Tarnawski AS||Journal of gastroenterology and hepatology (30 Suppl 1:85)||2015|
Mitochondrial DNA toxicity compromises mitochondrial dynamics and induces hippocampal antioxidant defenses.
459600 was used in immunohistochemistry - paraffin section to investigate the effects of mitochondrial DNA damage on hippocampal neurons.
|Lauritzen KH,Cheng C,Wiksen H,Bergersen LH,Klungland A||DNA repair (10:639)||2011|
EMG-normalised kinase activation during exercise is higher in human gastrocnemius compared to soleus muscle.
459600 was used in western blot to study the greater activation of phosphorylation pathways in human gastrocnemius as compared to soleus muscle when normalized for EMG activity.
|Jensen TE,Leutert R,Rasmussen ST,Mouatt JR,Christiansen ML,Jensen BR,Richter EA||PloS one (7:null)||2012|
GDP and carboxyatractylate inhibit 4-hydroxynonenal-activated proton conductance to differing degrees in mitochondria from skeletal muscle and heart.
459600 was used in western blot to investigate the differences of proton conductance mechanism between skeletal muscle and heart
|Aguirre E,Cadenas S||Biochimica et biophysica acta (1797:1716)||2010|
COX IV-1; COX4; COX4-1; COXIV; cytochrome c oxidase polypeptide IV; cytochrome c oxidase subunit 4 isoform 1 mitochondrial; cytochrome c oxidase subunit 4 isoform 1, mitochondrial; cytochrome c oxidase subunit IV; cytochrome c oxidase, subunit 4a; cytochrome c oxidase, subunit IV; cytochrome c oxidase, subunit IVa; cytochrome c oxydase subunit 4
AL024441; BOS_17018; COX; COX IV-1; COX4; COX4-1; Cox4a; COX4I1; COXIV; COXIV-1; IV-1