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|Tested species reactivity||Human , Zebra fish , Mouse , Rat , Cattle|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Bovine Complex IV (native holoenzyme protein, purified from liver).|
|Storage buffer||HEPES buffered saline|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Autophagy exacerbates caspase-dependent apoptotic cell death after short times of starvation.
A21348 was used in western blot to discuss the role of autophagy in cell death.
|Mattiolo P,Yuste VJ,Boix J,Ribas J||Biochemical pharmacology (98:573)||2015|
Mitochondria-associated microRNAs in rat hippocampus following traumatic brain injury.
A21348 was used in western blot to investigate the role of mitochondria-associated miRNAs in traumatic brain injury
|Wang WX,Visavadiya NP,Pandya JD,Nelson PT,Sullivan PG,Springer JE||Experimental neurology (265:84)||2015|
Insulin resistance and white adipose tissue inflammation are uncoupled in energetically challenged Fsp27-deficient mice.
A21348 was used in western blot to investigate the role of Fsp27 in adipose inflammation and hepatic insulin resistance
|Zhou L,Park SY,Xu L,Xia X,Ye J,Su L,Jeong KH,Hur JH,Oh H,Tamori Y,Zingaretti CM,Cinti S,Argente J,Yu M,Wu L,Ju S,Guan F,Yang H,Choi CS,Savage DB,Li P||Nature communications (6:null)||2015|
High-fat diet-induced impairment of skeletal muscle insulin sensitivity is not prevented by SIRT1 overexpression.
A21348 was used in western blot to test if constitutive activation of SIRT1 in skeletal muscle prevents high fat diet-induced muscle insulin resistance
|White AT,Philp A,Fridolfsson HN,Schilling JM,Murphy AN,Hamilton DL,McCurdy CE,Patel HH,Schenk S||American journal of physiology. Endocrinology and metabolism (307:E764)||2014|
Effect of high-intensity exercise on aged mouse brain mitochondria, neurogenesis, and inflammation.
A21348 was used in western blot to determine how intensive exercise affects brain bioenergetics, inflammation, and neurogenesis-relevant parameters
|E L,Burns JM,Swerdlow RH||Neurobiology of aging (35:2574)||2014|
Mitochondrial remodeling in hepatic differentiation and dedifferentiation.
A21348 was used in western blot to describe mitochondrial biogenesis during hepatogenic differentiation of bone marrow-mesenchymal stem cells
|Wanet A,Remacle N,Najar M,Sokal E,Arnould T,Najimi M,Renard P||The international journal of biochemistry & cell biology (54:174)||2014|
Perinatal protein malnutrition affects mitochondrial function in adult and results in a resistance to high fat diet-induced obesity.
A21348 was used in western blot to study the effect of perinatal protein malnutrition on mitochondrial function and obesity in adults
|Jousse C,Muranishi Y,Parry L,Montaurier C,Even P,Launay JM,Carraro V,Maurin AC,Averous J,Chaveroux C,Bruhat A,Mallet J,Morio B,Fafournoux P||PloS one (9:null)||2014|
Activation of the ubiquitin-proteasome system against arsenic trioxide cardiotoxicity involves ubiquitin ligase Parkin for mitochondrial homeostasis.
A21348 was used in western blot to assess the role of Parkin against cardiotoxicity elicited by arsenic trioxide exposure in HL-1 mouse atrial cardiomyocytes
|Watanabe M,Funakoshi T,Unuma K,Aki T,Uemura K||Toxicology (322:43)||2014|
Ubiquitin-specific protease 2a stabilizes MDM4 and facilitates the p53-mediated intrinsic apoptotic pathway in glioblastoma.
A21348 was used in western blot to study the relationship between USP2-MDM4 and p53 in tumorigenesis
|Wang CL,Wang JY,Liu ZY,Ma XM,Wang XW,Jin H,Zhang XP,Fu D,Hou LJ,Lu YC||Carcinogenesis (35:1500)||2014|
Altering O-linked β-N-acetylglucosamine cycling disrupts mitochondrial function.
A21348 was used in western blot to study the effect of O-GlcNAc cycling in mitochondria
|Tan EP,Villar MT,E L,Lu J,Selfridge JE,Artigues A,Swerdlow RH,Slawson C||The Journal of biological chemistry (289:14719)||2014|
The Aryl-Hydrocarbon Receptor Protein Interaction Network (AHR-PIN) as Identified by Tandem Affinity Purification (TAP) and Mass Spectrometry.
A21348 was used in western blot to define the aryl-hydrocarbon receptor-protein interaction network.
|Tappenden DM,Hwang HJ,Yang L,Thomas RS,Lapres JJ||Journal of toxicology (2013:null)||2014|
The role of PARL and HtrA2 in striatal neuronal injury after transient global cerebral ischemia.
A21348 was used in western blot to study the involvement of PARL and HtrA2 in striatal neuronal injury after transient global cerebral ischemia
|Yoshioka H,Katsu M,Sakata H,Okami N,Wakai T,Kinouchi H,Chan PH||Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (33:1658)||2013|
Shared and cell type-specific mitochondrial defects and metabolic adaptations in primary cells from PINK1-deficient mice.
A21348 was used in western blot to study the contribution of PINK to mitochondrial function/dynamics.
|Akundi RS,Zhi L,Sullivan PG,Büeler H||Neuro-degenerative diseases (12:136)||2013|
Striated muscle activator of Rho signaling is required for myotube survival but does not influence basal protein synthesis or degradation.
A21348 was used in western blot to test if STARS influences C2C2 myotube growth by regulating protein synthesis and degradation.
|Wallace MA,Russell AP||American journal of physiology. Cell physiology (305:C414)||2013|
β-Adrenergic stimulation does not activate p38 MAP kinase or induce PGC-1α in skeletal muscle.
A21348 was used in western blot to test if the increases in PGC-α and mitochondrial biogenesis induced by endurance exercise are mediated by catecholamines.
|Kim SH,Asaka M,Higashida K,Takahashi Y,Holloszy JO,Han DH||American journal of physiology. Endocrinology and metabolism (304:E844)||2013|
Sirtuin 1-mediated effects of exercise and resveratrol on mitochondrial biogenesis.
A21348 was used in western blot to determine the role of sirtuin in exercise- and resveratrol-induced skeletal muscle mitochondrial biogenesis.
|Menzies KJ,Singh K,Saleem A,Hood DA||The Journal of biological chemistry (288:6968)||2013|
Effect of exercise on mouse liver and brain bioenergetic infrastructures.
A21348 was used in western blot to examine the effects of exercise on liver and brain bioenergetic infrastructures.
|E L,Lu J,Burns JM,Swerdlow RH||Experimental physiology (98:207)||2013|
Inhibition of complex I regulates the mitochondrial permeability transition through a phosphate-sensitive inhibitory site masked by cyclophilin D.
A21348 was used in western blot to study the effect of different inhibitors on mitochondrial permeability transition pore.
|Li B,Chauvin C,De Paulis D,De Oliveira F,Gharib A,Vial G,Lablanche S,Leverve X,Bernardi P,Ovize M,Fontaine E||Biochimica et biophysica acta (1817:1628)||2012|
Shifting the paradigm: the putative mitochondrial protein ABCB6 resides in the lysosomes of cells and in the plasma membrane of erythrocytes.
A21348 was used in western blot to question the expression and function of ABCB6 in the mitochondria.
|Kiss K,Brozik A,Kucsma N,Toth A,Gera M,Berry L,Vallentin A,Vial H,Vidal M,Szakacs G||PloS one (7:null)||2012|
Oxidative capacity and fatigability in run-trained malignant hyperthermia-susceptible mice.
A21348 was used in western blot to test if the malignant hyperthermia model mice are more vulnerable to exercise-induced muscle injury and fatigability.
|Rouviere C,Corona BT,Ingalls CP||Muscle & nerve (45:586)||2012|
Mannitol-facilitated perfusion staining with 2,3,5-triphenyltetrazolium chloride (TTC) for detection of experimental cerebral infarction and biochemical analysis.
A21348 was used in western blot to describe a method to label severely injured or soft newborn brains using rodents.
|Sun YY,Yang D,Kuan CY||Journal of neuroscience methods (203:122)||2012|
Developmentally dynamic colocalization patterns of DSCAM with adhesion and synaptic proteins in the mouse retina.
A21348 was used in immunohistochemistry to study the role of down syndrome cell adhesion molecule during mice retina development
|de Andrade GB,Kunzelman L,Merrill MM,Fuerst PG||Molecular vision (20:1422)||2014|
COX4, COX4-1, COXIV, COX IV-1, cytochrome c oxidase polypeptide IV, cytochrome c oxidase subunit 4 isoform 1 mitochondrial